Designer receptors for nucleotide-resolution analysis of genomic 5-Methylcytosine by cellular imaging

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dc.contributor.authorMuñoz-López, Álvaro
dc.contributor.authorBuchmuller, Benjamin
dc.contributor.authorWolffgramm, Jan
dc.contributor.authorJung, Anne
dc.contributor.authorHussong, Michelle
dc.contributor.authorKanne, Julian
dc.contributor.authorSchweiger, Michal Ruth
dc.contributor.authorSummerer, Daniel
dc.date.accessioned2021-08-02T11:47:21Z
dc.date.available2021-08-02T11:47:21Z
dc.date.issued2020-03-13
dc.description.abstractWe report programmable receptors for the imaging-based analysis of 5-methylcytosine (5mC) in user-defined DNA sequences of single cells. Using fluorescent transcription-activator-like effectors (TALEs) that can recognize sequences of canonical and epigenetic nucleobases through selective repeats, we imaged cellular SATIII DNA, the origin of nuclear stress bodies (nSB). We achieve high nucleobase selectivity of natural repeats in imaging and demonstrate universal nucleobase binding by an engineered repeat. We use TALE pairs differing in only one such repeat in co-stains to detect 5mC in SATIII sequences with nucleotide resolution independently of differences in target accessibility. Further, we directly correlate the presence of heat shock factor 1 with 5mC at its recognition sequence, revealing a potential function of 5mC in its recruitment as initial step of nSB formation. This opens a new avenue for studying 5mC functions in chromatin regulation in situ with nucleotide, locus, and cell resolution.en
dc.identifier.urihttp://hdl.handle.net/2003/40364
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-22239
dc.language.isoende
dc.relation.ispartofseriesAngewandte Chemie International Edition;Vol. 59. 2020, issue 23, pp 8927-8931
dc.relation.replaceshttp://hdl.handle.net/2003/39224
dc.subjectBiosensorsen
dc.subjectDNA methylationen
dc.subjectEpigeneticsen
dc.subjectImaging probesen
dc.subjectMembrane-less organellesen
dc.subject.ddc570
dc.subject.ddc540
dc.subject.rswkBiosensorde
dc.subject.rswkDNSde
dc.subject.rswkMethylierungde
dc.subject.rswkEpigenetikde
dc.subject.rswkFluoreszenz-in-situ-Hybridisierungde
dc.subject.rswkOrganellde
dc.titleDesigner receptors for nucleotide-resolution analysis of genomic 5-Methylcytosine by cellular imagingen
dc.typeTextde
dc.type.publicationtypearticlede
dcterms.accessRightsopen access
eldorado.secondarypublicationtruede
eldorado.secondarypublication.primarycitationAngewandte Chemie International Edition. Vol. 59. 2020, issue 23, pp 8927-8931de
eldorado.secondarypublication.primaryidentifierhttps://doi.org/10.1002/anie.202001935de

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