Towards the design and construction of self-replicating RNA nanostructures
| dc.contributor.advisor | Mutschler, Hannes | |
| dc.contributor.author | Rubert, David | |
| dc.contributor.referee | Summerer, Daniel | |
| dc.date.accepted | 2024-05-10 | |
| dc.date.accessioned | 2024-08-02T13:18:32Z | |
| dc.date.available | 2024-08-02T13:18:32Z | |
| dc.date.issued | 2024 | |
| dc.description.abstract | The transition from an RNA-based world to DNA as the primary genetic material is a pivotal topic in origin-of-life research. This study aims to prototype a hybrid genome system combining DNA and RNA, capable of self-replication and evolution, initially in vitro and potentially in vivo. Using Qß replicase, segmented RNA genomes were synthesized, incorporating the Phi29 phage pRNA for self-assembly into nanorings. The replication efficacy of these replicons was evaluated both independently and in coupled assembly-replication reactions, with Qß chosen for its high amplification efficiency and template versatility. To mitigate the challenge of parasite RNA formation, which depletes essential replication components, a water-emulsion system was employed. The modular assembly of replicons into nanorings was further investigated, with a focus on system reproducibility and the effect of Mg2+ concentrations on nanoring stability. The final goal is to integrate self-replication with self-assembly, thereby creating a segmented, modular RNA genome for in vitro genetic information storage. This work addresses key challenges and advancements in developing synthetic self-replication systems for eventual genomic transplantation in E. coli. | en |
| dc.identifier.uri | http://hdl.handle.net/2003/42633 | |
| dc.identifier.uri | http://dx.doi.org/10.17877/DE290R-24469 | |
| dc.language.iso | en | de |
| dc.subject | RNA | en |
| dc.subject | Self-replicating RNA | en |
| dc.subject | Cell-free | en |
| dc.subject | pRNA | en |
| dc.subject.ddc | 570 | |
| dc.subject.ddc | 540 | |
| dc.subject.rswk | RNS | de |
| dc.title | Towards the design and construction of self-replicating RNA nanostructures | en |
| dc.type | Text | de |
| dc.type.publicationtype | PhDThesis | de |
| dcterms.accessRights | open access | |
| eldorado.secondarypublication | false | de |