Auswirkung hoher hydrostatischer Drücke auf Modellipidmembranen, Proteine und proteinmimetische Polymere
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2010-02-16T09:32:26Z
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In dieser Arbeit wurden die Auswirkungen hohen hydrostatischen Drucks auf Modellipidmembranen, Proteine und proteinmimetische Polymere/Hydrogele untersucht. Als Modellbiomembranen kamen die Gemische DPPC-Ergosterin (78:22 mol-%) und DOPC-DPPC-Cholesterin (1:2:1), jeweils in Überschußwasser, zum Einsatz, deren thermotrope und barotrope Phasenübergänge infrarotspektroskopisch analysiert wurden. Hieraus wurden entsprechende p,T-Phasendiagramme erstellt. Ferner wurde die Temperatur- und Druckstabilität der Peroxidase MsP1 aus dem Basidomyceten Marasmius scorodonius mit verschiedenen spektroskopischen Techniken untersucht und außerdem die Druckstabilität der Ankyrindomäne des Notch-Rezeptors aus Drosophila melanogaster in Abhängigkeit verschiedener Konzentrationen von 13C-Harnstoff infrarotspektroskopisch bestimmt. Abschließend wurden Polymere und Mikrogele aus Poly(N-isopropylacrylamid) und Poly(N,N-diethylacrylamid) auf ihr Temperatur- und Druckverhalten mittels Infrarotspektroskopie getestet.
In this doctoral thesis, the effects of high hydrostatic pressure on model lipid membranes, proteins and protein-mimetic polymers/hydrogels were investigated. As model membranes, the mixtures DPPC-Ergosterol (78:22 mol-%) and DOPC-DPPC-Cholesterol (1:2:1), both in excess water, were used and the respective thermotropic and barotropic phase transitions determined via infrared spectroscopy. Using all the obtained data, it was possible to establish p,T-phase diagrams for both mixtures. Furthermore, the temperature and pressure stability of the peroxidase MsP1 from the basidomycete fungus Marasmius scorodonius was explored using various spectroscopic techniques. In addition, the pressure stability of the ankyrin domain of the Notch receptor from Drosophila melanogaster in dependence of the concentration of 13C-urea was examined by infrared spectroscopy. Finally the temperature and pressure behaviour of polymers and microgels consisting of poly(N-isopropylacrylamide) and poly(N,N-diethylacrylamide) was studied via infrared spectroscopic analysis.
In this doctoral thesis, the effects of high hydrostatic pressure on model lipid membranes, proteins and protein-mimetic polymers/hydrogels were investigated. As model membranes, the mixtures DPPC-Ergosterol (78:22 mol-%) and DOPC-DPPC-Cholesterol (1:2:1), both in excess water, were used and the respective thermotropic and barotropic phase transitions determined via infrared spectroscopy. Using all the obtained data, it was possible to establish p,T-phase diagrams for both mixtures. Furthermore, the temperature and pressure stability of the peroxidase MsP1 from the basidomycete fungus Marasmius scorodonius was explored using various spectroscopic techniques. In addition, the pressure stability of the ankyrin domain of the Notch receptor from Drosophila melanogaster in dependence of the concentration of 13C-urea was examined by infrared spectroscopy. Finally the temperature and pressure behaviour of polymers and microgels consisting of poly(N-isopropylacrylamide) and poly(N,N-diethylacrylamide) was studied via infrared spectroscopic analysis.
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Hoher hydrostatischer Druck, Infrarotspektroskopie, Fluoreszenzspektroskopie, Modellbiomembran, Proteine, Hydrogele