Comparative proteomics analysis of Neisseria gonorrhoeae strains in response to extended-spectrum cephalosporins
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Date
2017-11-08
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Abstract
Neisseria gonorrhoeae strains displaying reduced susceptibility and resistance to extended-spectrum
cephalo- sporins (ESCs) are major public health concerns. Although resistance mechanisms of ESCs
have extensively been studied, the proteome-wide investigation on the biological response to the
antibiotic stress is still limited. Herein, a proteomics approach based on two-dimensional gel
electrophoresis and MALDI-TOF/TOF-MS analy- sis was applied to investigate the global protein
expression under ESC stresses of ESC-susceptible and ESC- reduced susceptible N. gonorrhoeae
strains. Upon exposure to ceftriaxone, 14 and 21 proteins of ESC- susceptible and ESC-reduced
susceptible strains, respectively, were shown to be differentially expressed. In the meanwhile,
differential expressions of 13 and 17 proteins were detected under cefixime stress for ESC-
susceptible and ESC-reduced susceptible strains, respectively. ESC antibiotics have been proven to
trigger the expression of several proteins implicated in a variety of biological functions
including transport system, energy metabolism, stress response and pathogenic virulence factors.
Interestingly, macrophage infectivity potentiators (Ng-MIP) showed increased expression for
ESC-reduced susceptible strain under ESC stress. The altered ex- pression of Ng-MIP was found to be
a unique response to ESC stresses. Our finding proposes a broad view on proteomic changes in N.
gonorrhoeae in response to ESC antibiotics that provides further insights into the gono-
coccal antimicrobial resistance and physiological adaptation mechanism.
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Keywords
Neisseria gonorrhoeae, Extended-spectrum cephalosporins, Antimicrobial resistance, Proteomics