Comparative proteomics analysis of Neisseria gonorrhoeae strains in response to extended-spectrum cephalosporins

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dc.contributor.authorNabu, Sunanta
dc.contributor.authorLawung, Ratana
dc.contributor.authorIsarankura-Na-Ayudhya, Patcharee
dc.contributor.authorRoytrakul, Sittiruk
dc.contributor.authorDolprasit, Supamas
dc.contributor.authorSengyee, Sineenart
dc.contributor.authorIsarankura-Na-Ayudhya, Chartchalerm
dc.contributor.authorPrachayasittikul, Virapong
dc.date.accessioned2018-02-05T12:45:22Z
dc.date.available2018-02-05T12:45:22Z
dc.date.issued2017-11-08
dc.description.abstractNeisseria gonorrhoeae strains displaying reduced susceptibility and resistance to extended-spectrum cephalo- sporins (ESCs) are major public health concerns. Although resistance mechanisms of ESCs have extensively been studied, the proteome-wide investigation on the biological response to the antibiotic stress is still limited. Herein, a proteomics approach based on two-dimensional gel electrophoresis and MALDI-TOF/TOF-MS analy- sis was applied to investigate the global protein expression under ESC stresses of ESC-susceptible and ESC- reduced susceptible N. gonorrhoeae strains. Upon exposure to ceftriaxone, 14 and 21 proteins of ESC- susceptible and ESC-reduced susceptible strains, respectively, were shown to be differentially expressed. In the meanwhile, differential expressions of 13 and 17 proteins were detected under cefixime stress for ESC- susceptible and ESC-reduced susceptible strains, respectively. ESC antibiotics have been proven to trigger the expression of several proteins implicated in a variety of biological functions including transport system, energy metabolism, stress response and pathogenic virulence factors. Interestingly, macrophage infectivity potentiators (Ng-MIP) showed increased expression for ESC-reduced susceptible strain under ESC stress. The altered ex- pression of Ng-MIP was found to be a unique response to ESC stresses. Our finding proposes a broad view on proteomic changes in N. gonorrhoeae in response to ESC antibiotics that provides further insights into the gono- coccal antimicrobial resistance and physiological adaptation mechanism.en
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/36356
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-18357
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal;Vol. 16 2017
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectNeisseria gonorrhoeaeen
dc.subjectExtended-spectrum cephalosporinsen
dc.subjectAntimicrobial resistanceen
dc.subjectProteomicsen
dc.subject.ddc610
dc.titleComparative proteomics analysis of Neisseria gonorrhoeae strains in response to extended-spectrum cephalosporinsen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.secondarypublicationtrue

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