Ectopic microRNAs used to preserve human mesenchymal stem cell potency and epigenetics

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dc.contributor.authorGhasemzadeh, Navid
dc.contributor.authorPourrajab, Fatemeh
dc.contributor.authorDehghani Firoozabadi, Ali
dc.contributor.authorHekmatimoghaddam, Seyedhossein
dc.contributor.authorHaghiralsadat, Fatemeh
dc.date.accessioned2018-11-08T13:58:19Z
dc.date.available2018-11-08T13:58:19Z
dc.date.issued2018-06-14
dc.description.abstractHuman mesenchymal stem cells (hMSCs) have remarkable potential for use in regenerative medicine. However, one of the great challenges is preserving their potency for long time. This study investigated the effect of miRNA ectopic expression on their proliferation and also on the expression level of Parp1 as an epigenetic switch preserving pluripotency in hMSCs. A cationic liposome was prepared as an efficient carrier for miRNA delivery. The miRNA loading efficiency and physical stability of vesicles were measured, and their scanning electron microscopic shapes determined. hMSCs were transfected with miR-302a and miR-34a followed by assessment of their proliferation potency with MTT assay and measurement of the expression of Parp1 by quantitative polymerase chain reaction (QPCR). Cell transfection with miR-302a and miR-34a efficiently and differentially affects the proliferation potency of hMSCs and the expression level of Parp1 as the key epigenetic factor involved in pluripotency. While miR-302a increases Parp1 expression, miR-34a suppresses it significantly, showing differential effects. Our results demonstrated that miRNA-based treatments represent efficient therapeutic systems and hold a great promise for future use in regenerative medicine through modification of hMSC pluripotency and epigenome.en
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/37646
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-19641
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal;Vol. 17 2018
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectEpigeneticen
dc.subjectHuman mesenchymal stem cells (hMSCs)en
dc.subjectmiRNAen
dc.subjectPotencyen
dc.subjectRegenerative medicineen
dc.subject.ddc610
dc.titleEctopic microRNAs used to preserve human mesenchymal stem cell potency and epigeneticsen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.secondarypublicationtrue

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