Ectopic microRNAs used to preserve human mesenchymal stem cell potency and epigenetics
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Date
2018-06-14
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Abstract
Human mesenchymal stem cells (hMSCs) have remarkable potential for use in regenerative medicine. However,
one of the great challenges is preserving their potency for long time. This study investigated the effect of miRNA
ectopic expression on their proliferation and also on the expression level of Parp1 as an epigenetic switch preserving
pluripotency in hMSCs. A cationic liposome was prepared as an efficient carrier for miRNA delivery.
The miRNA loading efficiency and physical stability of vesicles were measured, and their scanning electron microscopic
shapes determined. hMSCs were transfected with miR-302a and miR-34a followed by assessment of
their proliferation potency with MTT assay and measurement of the expression of Parp1 by quantitative polymerase
chain reaction (QPCR). Cell transfection with miR-302a and miR-34a efficiently and differentially affects
the proliferation potency of hMSCs and the expression level of Parp1 as the key epigenetic factor involved
in pluripotency. While miR-302a increases Parp1 expression, miR-34a suppresses it significantly, showing differential
effects. Our results demonstrated that miRNA-based treatments represent efficient therapeutic systems
and hold a great promise for future use in regenerative medicine through modification of hMSC pluripotency and
epigenome.
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Keywords
Epigenetic, Human mesenchymal stem cells (hMSCs), miRNA, Potency, Regenerative medicine