Acute exercise impacts AhR and PD-1 levels of CD8+ T-cells

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dc.contributor.authorSchenk, Alexander
dc.contributor.authorJoisten, Niklas
dc.contributor.authorWalzik, David
dc.contributor.authorKoliamitra, Christina
dc.contributor.authorSchoser, Daria
dc.contributor.authorBloch, Wilhelm
dc.contributor.authorZimmer, Philipp
dc.date.accessioned2021-04-15T05:33:12Z
dc.date.available2021-04-15T05:33:12Z
dc.date.issued2020-11-19
dc.description.abstractPurpose: The programmed cell death protein 1 (PD-1) has become a promising target in cancer immunotherapy. PD-1 expression of CD8+ T-cells may be increased via the exploitation of aryl hydrocarbon receptor (AhR) signaling with kynurenine (KYN) as a ligand. Since exercise affects KYN metabolism, we exploratory investigated the influence of acute exercise bouts on AhR and PD-1 levels of CD8+ T-cells. Method: In this study, 24 healthy males (age: 24.6 ± 3.9 years; weight 83.9 ± 10.5 kg; height: 182.4 ± 6.2 cm) completed a single bout of endurance (EE) and resistance exercise (RE) in a randomly assigned order on separate days. Blood samples were drawn before (t0), after (t1), and 1 h after (t2) both conditions. T-cell populations, the level of cytoplasmic AhR, and surface PD-1 were assessed by flow cytometry. Results: T-cell populations changed over time, indicated by an increase in the absolute numbers of CD3+ lymphocytes after EE (p < .001) and RE (p = .036) and in PD-1+ CD8+ T-cells after EE (p = .021). Proportions of T-cell populations changed only after EE (t0–t2: p = .029; t1-t2: p = .006). The level of cytoplasmic AhR decreased immediately after exercise in both exercise conditions (EE: p = .009; RE: p = .036). The level of surface PD-1 decreased 1 h after EE (p = .005). Conclusion: We analyzed the level of surface PD-1 and cytoplasmic AhR following acute physical exercise for the first time. Especially EE was observed to impact both AhR and PD-1 levels, undermining its role as the AhR-PD-1 axis modulator. These results provide new insights into the impact of exercise on AhR-signaling, which could potentially be relevant for various chronic diseases.en
dc.identifier.urihttp://hdl.handle.net/2003/40152
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-22024
dc.language.isoende
dc.relation.ispartofseriesEur J Appl Physiol;121
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAcute exerciseen
dc.subjectT-cellen
dc.subjectAhRen
dc.subjectPD-1en
dc.subjectKynurenineen
dc.subject.ddc796
dc.subject.rswkKynureninde
dc.titleAcute exercise impacts AhR and PD-1 levels of CD8+ T-cellsen
dc.title.alternativeExploratory results from a randomized cross-over trial comparing endurance versus resistance exerciseen
dc.typeTextde
dc.type.publicationtypearticlede
dcterms.accessRightsopen access
eldorado.secondarypublicationtruede
eldorado.secondarypublication.primarycitationSchenk, A., Joisten, N., Walzik, D. et al. Acute exercise impacts AhR and PD-1 levels of CD8+ T-cells—Exploratory results from a randomized cross-over trial comparing endurance versus resistance exercise. Eur J Appl Physiol 121, 637–644 (2021).de
eldorado.secondarypublication.primaryidentifierhttps://doi.org/10.1007/s00421-020-04552-wde

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