Effects of Trichostatin A on drug uptake transporters in primary rat hepatocyte cultures

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dc.contributor.authorRamboer, Eva
dc.contributor.authorRogiers, Vera
dc.contributor.authorVanhaecke, Tamara
dc.contributor.authorVinken, Mathieu
dc.date.accessioned2016-05-23T08:47:58Z
dc.date.available2016-05-23T08:47:58Z
dc.date.issued2015-05-05
dc.description.abstractThe present study was set up to investigate the effects of Trichostatin A (TSA), a prototypical epigenetic modifier, on the expression and activity of hepatic drug uptake transporters in primary cultured rat hepatocytes. To this end, the expression of the sinusoidal transporters sodium-dependent taurocholate cotransporting polypeptide (Ntcp) and organic anion transporting polypeptide 4 (Oatp4) was monitored by real-time quantitative reverse transcriptase polymerase chain reaction analysis and immunoblotting. The activity of the uptake transporters was analyzed using radiolabeled substrates and chemical inhibitors. Downregulation of the expression and activity of Oatp4 and Ntcp was observed as a function of the cultivation time and could not be counteracted by TSA. In conclusion, the epigenetic modifier TSA does not seem to exert a positive effect on the expression and activity of the investigated uptake transporters in primary rat hepatocyte cultures.en
dc.identifier.doi10.17179/excli2015-220
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/35001
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-17049
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal;Vol. 14, 2015en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectepigeneticsen
dc.subjectTrichostatin Aen
dc.subjectdrug transportersen
dc.subjectprimary hepatocyte cultureen
dc.subject.ddc610
dc.titleEffects of Trichostatin A on drug uptake transporters in primary rat hepatocyte culturesen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1

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