Quantitation of zolpidem in biological fluids by electro-driven microextraction combined with HPLC-UV analysis

Simple item page
dc.contributor.authorYaripour, Saeid
dc.contributor.authorMohammadi, Ali
dc.contributor.authorEsfanjani, Isa
dc.contributor.authorWalker, Roderick B
dc.contributor.authorNojavan, Saeed
dc.date.accessioned2018-07-26T10:40:00Z
dc.date.available2018-07-26T10:40:00Z
dc.date.issued2018-04-23
dc.description.abstractIn this study, for the first time, an electro-driven microextraction method named electromembrane extraction combined with a simple high performance liquid chromatography and ultraviolet detection was developed and validated for the quantitation of zolpidem in biological samples. Parameters influencing electromembrane extraction were evaluated and optimized. The membrane consisted of 2-ethylhexanol immobilized in the pores of a hollow fiber. As a driving force, a 150 V electric field was applied to facilitate the analyte migration from the sample matrix to an acceptor solution through a supported liquid membrane. The pHs of donor and acceptor solutions were optimized to 6.0 and 2.0, respectively. The enrichment factor was obtained >75 within 15 minutes. The effect of carbon nanotubes (as solid nano-sorbents) on the membrane performance and EME efficiency was evaluated. The method was linear over the range of 10-1000 ng/mL for zolpidem (R2 >0.9991) with repeatability ( %RSD) between 0.3 % and 7.3 % (n = 3). The limits of detection and quantitation were 3 and 10 ng/mL, respectively. The sensitivity of HPLC-UV for the determination of zolpidem was enhanced by electromembrane extraction. Finally, the method was employed for the quantitation of zolpidem in biological samples with relative recoveries in the range of 60–79 %.en
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/37072
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-19069
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal;Vol. 17 2018
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBiological fluidsen
dc.subjectElectromembrane extractionen
dc.subjectHPLC-UVen
dc.subjectQuantitationen
dc.subjectZolpidemen
dc.subject.ddc610
dc.titleQuantitation of zolpidem in biological fluids by electro-driven microextraction combined with HPLC-UV analysisen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.secondarypublicationtrue

Files

Original bundle
Now showing 1 - 3 of 3
Thumbnail Image
Name:
Mohammadi_23042018_proof.pdf
Size:
463.6 KB
Format:
Adobe Portable Document Format
Description:
DNB
Download
No Thumbnail Available
Name:
Mohammadi_23042018_supplementary_data.xls
Size:
151 KB
Format:
Microsoft Excel
Description:
DNB
Download
No Thumbnail Available
Name:
container.zip
Size:
471.31 KB
Format:
Zip-Archivdatei
Description:
DNB-Container
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
4.85 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Original Articles