Automatic quantitative analysis of morphology of apoptotic HL-60 cells
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Date
2014-01-29
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Abstract
Morphological identification is a widespread procedure to assess the presence of apoptosis by visual inspection of the morphological characteristics or the fluorescence images. The procedure is lengthy and results are observer dependent. A quantitative automatic analysis is objective and would greatly help the routine work. We developed an image processing and segmentation method which combined the
Otsu thresholding and morphological operators
for apoptosis study. An automatic determination method of apoptotic stages of HL-60 cells with fluorescence images was developed. Comparison was made between normal cells, early apoptotic cells and late apoptotic cells about their geometric parameters which were defined to describe the features of cell morphology. The results
demonstrated that the parameters we chose
are very representative of the morphologic
al characteristics of apoptotic cells.
Significant differences exist between the cells in different stages, and automatic quantification of the differences can be achieved.
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Keywords
cell morphology, quantitative analysis, apoptosis