Automatic quantitative analysis of morphology of apoptotic HL-60 cells

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dc.contributor.authorLiu, Yahui
dc.contributor.authorLin, Wang
dc.contributor.authorYang, Xu
dc.contributor.authorLiang, Weizi
dc.contributor.authorZhang, Jun
dc.contributor.authorMeng, Maobin
dc.contributor.authorRice, John R.
dc.contributor.authorSa, Yu
dc.contributor.authorFeng, Yuanming
dc.date.accessioned2014-05-13T11:37:42Z
dc.date.available2014-05-13T11:37:42Z
dc.date.issued2014-01-29
dc.description.abstractMorphological identification is a widespread procedure to assess the presence of apoptosis by visual inspection of the morphological characteristics or the fluorescence images. The procedure is lengthy and results are observer dependent. A quantitative automatic analysis is objective and would greatly help the routine work. We developed an image processing and segmentation method which combined the Otsu thresholding and morphological operators for apoptosis study. An automatic determination method of apoptotic stages of HL-60 cells with fluorescence images was developed. Comparison was made between normal cells, early apoptotic cells and late apoptotic cells about their geometric parameters which were defined to describe the features of cell morphology. The results demonstrated that the parameters we chose are very representative of the morphologic al characteristics of apoptotic cells. Significant differences exist between the cells in different stages, and automatic quantification of the differences can be achieved.en
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/33123
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-15476
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal ; Vol. 13, 2014en
dc.subjectcell morphologyen
dc.subjectquantitative analysisen
dc.subjectapoptosisen
dc.subject.ddc610
dc.titleAutomatic quantitative analysis of morphology of apoptotic HL-60 cellsen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1

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