Production, purification and characterization of novel beta glucosidase from newly isolated Penicillium simplicissimum H-11 in submerged fermentation
| dc.contributor.author | Bai, Hongzhi | |
| dc.contributor.author | Wang, Hui | |
| dc.contributor.author | Sun, Junde | |
| dc.contributor.author | Irfan, Muhammad | |
| dc.contributor.author | Han, Mei | |
| dc.contributor.author | Huang, Yuqian | |
| dc.contributor.author | Han, Xiaori | |
| dc.contributor.author | Yang, Qian | |
| dc.date.accessioned | 2014-03-10T10:33:48Z | |
| dc.date.available | 2014-03-10T10:33:48Z | |
| dc.date.issued | 2013-06-13 | |
| dc.description.abstract | β-Glucosidase is an important component of the cellulase complex. It not only hydrolyzes cellobiose and short-chain cellooligos accharides to glucose, but also removes the inhibitory effect of cellobiose on the β-1, 4-endoglucanase and exoglucanase, thereby increasing the overall rate of cellulose biodegradation. β-glucosidasefrom culture supernatant of a fungus Penicillium simplicissimum was purified to homogeneity, by using ammonium sulfate fraction, Sephadex G-100 chromatography, and its properties were studied. The molecular mass of the enzyme was about 126.0 kDa, as identified by 12% SDS-PAGE. The optimum pH and temperature were 4.4 ~ 5.2 and 60°C, respectively. The enzyme was stable in pH 5.2 ~ 6.4 and under 40°C. Metal profile of the enzyme showed that Mn2+ enhances its activity, while Cu2+, Co2+ and Fe3+ cause obvious inhibition. The Km and Vmax was 14.881 mg/ml and 0.364 mg ml/min against salicin as a Substrate. This enzyme had secondary protein structure as evidenced by FTIR spectrum. | en |
| dc.identifier.issn | 1611-2156 | |
| dc.identifier.uri | http://hdl.handle.net/2003/32936 | |
| dc.identifier.uri | http://dx.doi.org/10.17877/DE290R-7346 | |
| dc.language.iso | en | |
| dc.relation.ispartofseries | EXCLI Journal ; Vol. 12, 2013 | en |
| dc.subject | β -Glucosidase | en |
| dc.subject | Penicillium simplicissimum | en |
| dc.subject | purification | en |
| dc.subject | characterization | en |
| dc.subject.ddc | 610 | |
| dc.title | Production, purification and characterization of novel beta glucosidase from newly isolated Penicillium simplicissimum H-11 in submerged fermentation | en |
| dc.type | Text | |
| dc.type.publicationtype | article | |
| dcterms.accessRights | open access | |
| eldorado.dnb.zdberstkatid | 2132560-1 |