Authors: Suter, Dieter
Börger, Birgit
Gutschank, Jörg
Bingham, Stephen J.
Thomson, Andrew J.
Title: Deconvolution and assignment of different optical transitions in the blue copper protein azurin from optically detected electron paramagnetic resonance spectroscopy
Language (ISO): en
Abstract: Magnetic circular dichroism is a powerful spectroscopic tool for the assignment of optical resonance lines. An extension of this technique, microwave-modulated circular dichroism, provides additional details, in particular information about the orientation of optical transition moments. It arises from magnetization precessing around the static magnetic field, excited by a microwave field, in close analogy to electron paramagnetic resonance (EPR). In this paper we investigate the visible and near-infrared spectrum of the blue copper protein Pseudomonas aeruginosa azurin. Using a nonoriented sample (frozen solution), we apply this technique to measure the variation of the optical anisotropy with the wavelength. A comparison with the optical anisotropies of the possible ligand-field and charge-transfer transitions allows us to identify individual resonance lines in the strongly overlapping spectrum and assign them to specific electronic transitions. The technique is readily applicable to other proteins with transition metal centers.
URI: http://hdl.handle.net/2003/25355
http://dx.doi.org/10.17877/DE290R-2689
Publishers Link: http://e3.physik.tu-dortmund.de/~suter/eprints/JACS_ODPER_of_Azurin.pdf
Issue Date: 2001-02-15
Provenance: American Chemical Society
URL: http://dx.doi.org/10.1021/ja003357b
Citation: Börger, Birgit; Gutschank, Jörg; Suter, Dieter, Thomson, Andrew J.; Bingham, Stephen J.: Deconvolution and assignment of different optical transitions in the blue copper protein azurin from optically detected electron paramagnetic resonance spectroscopy. In: Journal of the American Chemical Society Nr. 10, Jg. 123(2001), S. 2334-2339
Appears in Collections:Suter, Dieter Prof. Dr.

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