Authors: | Guo, Jia Wang, Myeong-Hyeon |
Title: | Extract of Ulmus davidiana Planch barks induced apoptosis in human hepatoma cell line HepG2 |
Language (ISO): | en |
Abstract: | The cytotoxicity and mechanism of cell death induced by an extract of Ulmus davidiana Planch (UDP) were investigated. A 70 % ethanol extract of UDP exerted cytotoxic effects on HepG2 cells in a time- and dose-dependent manner caused a significant dose-dependent increase in the number of apoptotic cells. In addition, obvious shrinkage and destruction of the monolayer were observed in UDP-treated cells, but not in untreated cells. RT-PCR analysis revealed that the mRNA expression of p53 and c-myc was markedly increased in cells treated with the plant extract. In contrast, caspase-3 expression was induced after 1 h of incubation and then decreased at 3 h; however, it subsequently increased and remained at a relatively high level thereafter. Our data suggest the presence of a bioactive compound capable of killing liver carcinoma cells by apoptosis in a 70 % ethanol extract of UDP, and that the mechanism of apoptosis in the HepG2 cells included p53 and c-myc signaling. |
Subject Headings: | 70 % ethanol extract apoptosis HepG2 Ulmus davidiana Planch |
URI: | http://hdl.handle.net/2003/26221 http://dx.doi.org/10.17877/DE290R-7314 |
Issue Date: | 2009-07-08T07:27:52Z |
Appears in Collections: | Original Articles |
Files in This Item:
File | Description | Size | Format | |
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Guo_Wang_ethanol_extract_220609_proof.pdf | DNB | 494.7 kB | Adobe PDF | View/Open |
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