Authors: Ko, Eun-Yi
Lee, Seung-Hong
Ko, Ji-Yeon
Moon, Jeong Yong
Yoon, Weon-Jong
Ahn, Ginnae
Roh, Seong Woon
Cho, Kichul
Jeon, You-Jin
Kim, Daekyung
Kim, Kil-Nam
Title: Trans-1,3-diphenyl-2,3-epoxypropan-1-one, a chalcone derivative, induces apoptosis via ROS-mediated down-regulation of Bcl-xL in human leukemia HL-60 cells
Language (ISO): en
Abstract: The anticancer effects of trans-1,3-diphenyl-2,3-epoxypropan-1-one (DPEP), a chalcone derivative, were investigated in human leukemia HL-60 cells. Treatment of HL-60 cells with various concentration of DPEP resulted in a sequence of events characteristic of apoptosis, including loss of cell viability, morphological changes, and increased sub-G1 DNA content. We demonstrated that DPEP elevates reactive oxygen species (ROS) levels in HL-60 cells, and that the ROS scavenger N-acetylcysteine (NAC) could block DPEP-induced ROS generation and apoptosis. Western blot analysis revealed that DPEP inhibits Bcl-xL expression, leading to caspase-3 activation and poly-ADP-ribose polymerase (PARP) cleavage, thereby inducing apoptosis. However, NAC pretreatment significantly inhibited the activation of caspase-3 and PARP cleavage and reduced Bcl-xL levels. These findings provide the first evidence that DPEP may inhibit the growth of HL-60 cells and induce apoptosis through a ROS-mediated Bcl-xL pathway.
Subject Headings: anticancer
trans-1,3-diphenyl-2,3-epoxypropan-1-one (DPEP)
reactive oxygen species (ROS)
Issue Date: 2015-08-03
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