Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Yamkamon, Vichanan | - |
dc.contributor.author | Yee, Pyone Pyone | - |
dc.contributor.author | Yainoi, Sakda | - |
dc.contributor.author | Eiamphungporn, Warawan | - |
dc.contributor.author | Suksrichavalit, Thummaruk | - |
dc.date.accessioned | 2019-03-25T07:37:57Z | - |
dc.date.available | 2019-03-25T07:37:57Z | - |
dc.date.issued | 2018-10-16 | - |
dc.identifier.issn | 1611-2156 | - |
dc.identifier.uri | http://hdl.handle.net/2003/37954 | - |
dc.identifier.uri | http://dx.doi.org/10.17877/DE290R-19939 | - |
dc.description.abstract | Shortly after sarcosine was delineated as a potential biomarker for prostate cancer in 2009, a variety of analytical methods for clinical application were developed. Moreover, higher uptake of glycine in the mitochondria also played a role in cancer proliferation. A major constraint in the accurate quantification of sarcosine was the interference of the two isomers, α-alanine and β-alanine, using chromatographic separation techniques. Accordingly, we aimed to develop an analytical method for determining sarcosine and its related metabolites (α- and β-alanine, glycine and creatinine) under the same conditions by gas chromatography-tandem mass spectrometry (GCMS/ MS). BSTFA + 1 % TMCS was used for silylation, and GC-MS/MS conditions were optimized for the target analytes. The unique transition ions of sarcosine, α- and β-alanine, glycine and creatinine set up in MRM acquisition were m/z 116 → 73, 190 → 147, 176 → 147, 176 → 147 and 100 → 73, respectively. This newly developed method was successfully validated to apply in clinical settings with low limits of detection (0.01 - 0.03 μg•mL-1), high correlations (R2 > 0.99), great accuracy (88 – 110 % recovery), and notable precision (RSD < 10 %). All TMS derivatives were > 80 % stable for up to 2 h after derivatization and analyzing during this period promises to achieve an accurate result. Monitoring the five-substance profile could enhance prospects for early diagnosis of prostate cancer. | en |
dc.language.iso | en | - |
dc.relation.ispartofseries | EXCLI Journal;Vol. 17 2018 | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | Gas chromatography-tandem mass spectrometry | en |
dc.subject | Sarcosine | en |
dc.subject | Alanine | en |
dc.subject | Glycine | en |
dc.subject | Creatinine | en |
dc.subject | MRM | en |
dc.subject.ddc | 610 | - |
dc.title | Simultaneous determination of sarcosine and its related metabolites by gas chromatography-tandem mass spectrometry for prostate cancer diagnosis | en |
dc.type | Text | - |
dc.type.publicationtype | article | - |
dcterms.accessRights | open access | - |
eldorado.dnb.zdberstkatid | 2132560-1 | - |
eldorado.secondarypublication | true | - |
Appears in Collections: | Original Articles |
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File | Description | Size | Format | |
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Suksrichavalit_16102018_proof.pdf | DNB | 451.27 kB | Adobe PDF | View/Open |
Suksrichavalit_16102018_supplementary_data.xlsx | DNB | 452.64 kB | Microsoft Excel XML | View/Open |
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