Autor(en): | Yamkamon, Vichanan Yee, Pyone Pyone Yainoi, Sakda Eiamphungporn, Warawan Suksrichavalit, Thummaruk |
Titel: | Simultaneous determination of sarcosine and its related metabolites by gas chromatography-tandem mass spectrometry for prostate cancer diagnosis |
Sprache (ISO): | en |
Zusammenfassung: | Shortly after sarcosine was delineated as a potential biomarker for prostate cancer in 2009, a variety of analytical methods for clinical application were developed. Moreover, higher uptake of glycine in the mitochondria also played a role in cancer proliferation. A major constraint in the accurate quantification of sarcosine was the interference of the two isomers, α-alanine and β-alanine, using chromatographic separation techniques. Accordingly, we aimed to develop an analytical method for determining sarcosine and its related metabolites (α- and β-alanine, glycine and creatinine) under the same conditions by gas chromatography-tandem mass spectrometry (GCMS/ MS). BSTFA + 1 % TMCS was used for silylation, and GC-MS/MS conditions were optimized for the target analytes. The unique transition ions of sarcosine, α- and β-alanine, glycine and creatinine set up in MRM acquisition were m/z 116 → 73, 190 → 147, 176 → 147, 176 → 147 and 100 → 73, respectively. This newly developed method was successfully validated to apply in clinical settings with low limits of detection (0.01 - 0.03 μg•mL-1), high correlations (R2 > 0.99), great accuracy (88 – 110 % recovery), and notable precision (RSD < 10 %). All TMS derivatives were > 80 % stable for up to 2 h after derivatization and analyzing during this period promises to achieve an accurate result. Monitoring the five-substance profile could enhance prospects for early diagnosis of prostate cancer. |
Schlagwörter: | Gas chromatography-tandem mass spectrometry Sarcosine Alanine Glycine Creatinine MRM |
URI: | http://hdl.handle.net/2003/37954 http://dx.doi.org/10.17877/DE290R-19939 |
Erscheinungsdatum: | 2018-10-16 |
Rechte (Link): | https://creativecommons.org/licenses/by/4.0/ |
Enthalten in den Sammlungen: | Original Articles |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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Suksrichavalit_16102018_proof.pdf | DNB | 451.27 kB | Adobe PDF | Öffnen/Anzeigen |
Suksrichavalit_16102018_supplementary_data.xlsx | DNB | 452.64 kB | Microsoft Excel XML | Öffnen/Anzeigen |
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