Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Schachtsiek, Julia | - |
dc.contributor.author | Stehle, Felix | - |
dc.date.accessioned | 2019-10-04T11:03:04Z | - |
dc.date.available | 2019-10-04T11:03:04Z | - |
dc.date.issued | 2019-08-23 | - |
dc.identifier.uri | http://hdl.handle.net/2003/38262 | - |
dc.identifier.uri | http://dx.doi.org/10.17877/DE290R-20232 | - |
dc.description.abstract | This dataset in brief is related to the research letter entitled "Nicotine-free, nontransgenic tobacco (Nicotiana tabacuml.) edited by CRISPR-Cas9" [1]. Cured tobacco products with a significantly reduced nicotine content helps people to overcome their nicotine addiction. Here we summarize additional data and method descriptions of the generation process of a nicotine-free, nontransgenic tobacco plant. This included the cloning, transformation and regeneration of transgenic tobacco plants, followed by the analysis of the nicotine content and genomic modifications caused by CRISPR-Cas9 mediated gene editing. Subsequently, nicotine-free plants were screened for loss of T-DNA cassette, i.e. nontransgenity. Finally, a metabolic footprint was recorded by 1H NMR analysis. | de |
dc.language.iso | en | de |
dc.relation.ispartofseries | Data in Brief;26. 104395 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | CRISPR-Cas9 | de |
dc.subject | Nicotine-free | de |
dc.subject | Gene editing | de |
dc.subject | Nontransgenic | de |
dc.subject | Nicotiana tabacum | de |
dc.subject | Non-addictive | de |
dc.subject.ddc | 660 | - |
dc.title | Dataset on nicotine-free, nontransgenic tobacco (Nicotiana tabacum l.) edited by CRISPR-Cas9 | de |
dc.type | Text | de |
dc.type.publicationtype | article | de |
dcterms.accessRights | open access | - |
eldorado.secondarypublication | true | de |
eldorado.secondarypublication.primaryidentifier | DOI: 10.1016/j.dib.2019.104395 | de |
eldorado.secondarypublication.primarycitation | Data in Brief. 26. 2019, 104395 | de |
Appears in Collections: | Lehrstuhl Technische Biochemie |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Dataset_on_nicotine-free_nontransgenic_tobacco_Nic.pdf | 1.7 MB | Adobe PDF | View/Open |
This item is protected by original copyright |
This item is licensed under a Creative Commons License