Autor(en): | Schachtsiek, Julia Stehle, Felix |
Titel: | Dataset on nicotine-free, nontransgenic tobacco (Nicotiana tabacum l.) edited by CRISPR-Cas9 |
Sprache (ISO): | en |
Zusammenfassung: | This dataset in brief is related to the research letter entitled "Nicotine-free, nontransgenic tobacco (Nicotiana tabacuml.) edited by CRISPR-Cas9" [1]. Cured tobacco products with a significantly reduced nicotine content helps people to overcome their nicotine addiction. Here we summarize additional data and method descriptions of the generation process of a nicotine-free, nontransgenic tobacco plant. This included the cloning, transformation and regeneration of transgenic tobacco plants, followed by the analysis of the nicotine content and genomic modifications caused by CRISPR-Cas9 mediated gene editing. Subsequently, nicotine-free plants were screened for loss of T-DNA cassette, i.e. nontransgenity. Finally, a metabolic footprint was recorded by 1H NMR analysis. |
Schlagwörter: | CRISPR-Cas9 Nicotine-free Gene editing Nontransgenic Nicotiana tabacum Non-addictive |
URI: | http://hdl.handle.net/2003/38262 http://dx.doi.org/10.17877/DE290R-20232 |
Erscheinungsdatum: | 2019-08-23 |
Rechte (Link): | http://creativecommons.org/licenses/by/4.0/ |
Enthalten in den Sammlungen: | Lehrstuhl Technische Biochemie |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
---|---|---|---|---|
Dataset_on_nicotine-free_nontransgenic_tobacco_Nic.pdf | 1.7 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource ist urheberrechtlich geschützt. |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons