Authors: Barbeck, Mike
Jung, Ole
Xiong, Xin
Krastev, Rumen
Korzinskas, Tadas
Najman, Stevo
Radenković, Milena
Wegner, Nils
Knyazeva, Marina
Walther, Frank
Title: Balancing purification and ultrastructure of naturally derived bone blocks for bone regeneration
Other Titles: report of the purification effort of two bone blocks
Language (ISO): en
Abstract: The present publication reports the purification effort of two natural bone blocks, that is, an allogeneic bone block (maxgraft®, botiss biomaterials GmbH, Zossen, Germany) and a xenogeneic block (SMARTBONE®, IBI S.A., Mezzovico-Vira, Switzerland) in addition to previously published results based on histology. Furthermore, specialized scanning electron microscopy (SEM) and in vitro analyses (XTT, BrdU, LDH) for testing of the cytocompatibility based on ISO 10993-5/-12 have been conducted. The microscopic analyses showed that both bone blocks possess a trabecular structure with a lamellar subarrangement. In the case of the xenogeneic bone block, only minor remnants of collagenous structures were found, while in contrast high amounts of collagen were found associated with the allogeneic bone matrix. Furthermore, only island-like remnants of the polymer coating in case of the xenogeneic bone substitute seemed to be detectable. Finally, no remaining cells or cellular remnants were found in both bone blocks. The in vitro analyses showed that both bone blocks are biocompatible. Altogether, the purification level of both bone blocks seems to be favorable for bone tissue regeneration without the risk for inflammatory responses or graft rejection. Moreover, the analysis of the maxgraft® bone block showed that the underlying purification process allows for preserving not only the calcified bone matrix but also high amounts of the intertrabecular collagen matrix.
Subject Headings: Bone block
Bone regeneration
Subject Headings (RSWK): Knochenspan
Allogenes Transplantat
Issue Date: 2019-10-02
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Appears in Collections:Fachgebiet Werkstoffprüftechnik

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