Authors: Aliakbari, Sara
Mohammadi, Mobin
Rezaee, Mohammad Ali
Amini, Abbas Ali
Fakhari, Shohreh
Rahmani, Mohammad Reza
Title: Impaired immunomodulatory ability of type 2 diabetic adipose-derived mesenchymal stem cells in regulation of inflammatory condition in mixed leukocyte reaction
Language (ISO): en
Abstract: The immunomodulatory properties of type 2 diabetic patients’ adipose-derived mesenchymal stem cells (D-ASCs) has not been extensively studied. In this study, we compared the immunomodulatory properties of D-ASCs and non-diabetic subjects mesenchymal stem cells (ND-ASCs) in co-culture with mixed leukocyte reaction (MLR). ASCs were isolated from adipose tissue samples of type 2 diabetic and non-diabetic subjects (age: 40-55). D-ASCs and ND-ASCs were co-cultured with two-way MLR. Peri pheral blood mononuclear ce lls (PBMCs) proliferation ratio, protein levels of IFN- γ and IL-10, mRNA expression of COX-2 , TNF- α , TGF- β 1 and IL-6 genes in MLR, D-ASCs and ND-ASCs co-cultures were assessed using XTT, ELISA and Real-time qRT-PCR, respectively. PBMCs proliferation on days 2 and 4 of D-ASCs co-culture was higher than ND-ASCs co-culture of the same days ( p < 0.001). IFN- γ level decreased on day 4 compared to day 2 of ND-ASCs co-culture, but its level had not changed in D-ASCs co-culture. COX-2 expression on days 2 and 4 of D-ASCs co-culture was lower than ND- ASCs co-culture of the same days ( p < 0.05). The expression of TNF- α and IL-6 on days 2 and 4 of D-ASCs co- culture were higher than ND-ASCs co-culture of the same days ( p < 0.001). TGF- β 1 on day 4 of ND-ASCs co- culture showed a slightly higher expression than D-ASCs co-culture of the same day. Lower suppression of PBMCs proliferation, declined expre ssion of anti-inflammatory and upregulated expression of pro-inflammatory factors in D-ASCs co-culture, indicated an impairment of these cells in modulation of the inflammatory condition.
Subject Headings: Type 2 diabetes
Immunomodulation
Mesenchymal stem cells
Adipose tissue
Inflammation
URI: http://hdl.handle.net/2003/39053
http://dx.doi.org/10.17877/DE290R-20972
Issue Date: 2019-09-23
Appears in Collections:Original Articles

Files in This Item:
File Description SizeFormat 
Rahmani_23092019_proof.pdfDNB622.06 kBAdobe PDFView/Open


This item is protected by original copyright



All resources in the repository are protected by copyright.