Autor(en): | Guo, Zhenyu Huang, Shuchang Zhou, Yihong Cheng, Wenjie Chu, Xi Zheng, Xiaoqing Zheng, Hao |
Titel: | MicroRNA-9-5p functions as a tumor suppressor in prostate cancer via targeting UTRN |
Sprache (ISO): | en |
Zusammenfassung: | Accumulating evidence indicates that miR-9-5p plays an important role in several diseases, especially tumor progression. In this study, we investigated the clinical significance and biological function of miR-9-5p in prostate cancer (PCa). Using quantitative real time PCR (qRT-PCR) analysis, we found miR-9-5p level was significantly down-regulated in PCa tissues and cell lines. The decreased miR-9-5p expression was associated with tumor size, preoperative PSA, Gleason score and lymph node metastasis. Kaplan-Meier survival analysis showed patients with low level of miR-9-5p had significantly decreased rates of overall survival (OS). Multivariate analyses showed that miR-9-5p was an independent predictor of PCa patients’ prognosis. Through CCK-8 and Transwell assays, miR-9-5p overexpression by miR-9-5p mimics transfection was demonstrated to suppress the proliferation, migration and invasion of PCa cells. Mechanistically, luciferase reporter assay, qRT-PCR and Western blot demonstrated that Utrophin (UTRN) is a direct target of miR-9-5p in PCa cells. The status of UTRN protein in PCa tissues was much higher than that in adjacent tissues by immunohistochemical staining and its mRNA levels were inversely correlated with miR-9-5p in PCa tissues. Importantly, UTRN knockdown by siUTRN imitated the suppressive effects of miR-9-5p on cell proliferation, migration and invasion in PCa. In summary, miR-9-5p might novel prognostic biomarker in and targeting UTRN by miR-9-5p could be potential therapeutic candidates for PCa. |
Schlagwörter: | Prostate cancer miR-9-5P UTRN Prognosis Cell proliferation Migration |
URI: | http://hdl.handle.net/2003/39113 http://dx.doi.org/10.17877/DE290R-21031 |
Erscheinungsdatum: | 2019-11-07 |
Rechte (Link): | https://creativecommons.org/licenses/by/4.0/ |
Enthalten in den Sammlungen: | Original Articles |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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Zheng_Zheng_07112019_proof.pdf | DNB | 897.96 kB | Adobe PDF | Öffnen/Anzeigen |
Zheng_Zheng_07112019_supplementary_data.pdf | DNB | 194.95 kB | Adobe PDF | Öffnen/Anzeigen |
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