Autor(en): Schmitz, Lisa Marie
Kinner, Alina
Althoff, Kirsten
Rosenthal, Katrin
Lütz, Stephan
Titel: Investigation of vitamin D2 and vitamin D3 hydroxylation by Kutzneria albida
Sprache (ISO): en
Zusammenfassung: The active vitamin D metabolites 25-OH−D and 1α,25-(OH)2−D play an essential role in controlling several cellular processes in the human body and are potentially effective in the treatment of several diseases, such as autoimmune diseases, cardiovascular diseases and cancer. The microbial synthesis of vitamin D2 (VD2) and vitamin D3 (VD3) metabolites has emerged as a suitable alternative to established complex chemical syntheses. In this study, a novel strain, Kutzneria albida, with the ability to form 25-OH−D2 and 25-OH−D3 was identified. To further improve the conversion of the poorly soluble substrates, several solubilizers were tested. 100-fold higher product concentrations of 25-OH−D3 and tenfold higher concentrations of 25-OH−D2 after addition of 5 % (w/v) 2-hydroxypropyl β-cyclodextrin (2-HPβCD) were reached. Besides the single-hydroxylation products, the human double-hydroxylation products 1,25-(OH)2−D2 and 1,25-(OH)2−D3 and various other potential single- and double-hydroxylation products were detected. Thus, K. albida represents a promising strain for the biotechnological production of VD2 and VD3 metabolites.
Schlagwörter: Biotransformations
Cyclodextrins
Hydroxylation
Kutzneria albida
Vitamin D
URI: http://hdl.handle.net/2003/40852
http://dx.doi.org/10.17877/DE290R-22709
Erscheinungsdatum: 2021-03-01
Rechte (Link): https://creativecommons.org/licenses/by/4.0/
Enthalten in den Sammlungen:Arbeitsgruppe Bioverfahrenstechnik



Diese Ressource ist urheberrechtlich geschützt.



Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons Creative Commons