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dc.contributor.advisorMutschler, Hannes-
dc.contributor.authorRubert, David-
dc.date.accessioned2024-08-02T13:18:32Z-
dc.date.available2024-08-02T13:18:32Z-
dc.date.issued2024-
dc.identifier.urihttp://hdl.handle.net/2003/42633-
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-24469-
dc.description.abstractThe transition from an RNA-based world to DNA as the primary genetic material is a pivotal topic in origin-of-life research. This study aims to prototype a hybrid genome system combining DNA and RNA, capable of self-replication and evolution, initially in vitro and potentially in vivo. Using Qß replicase, segmented RNA genomes were synthesized, incorporating the Phi29 phage pRNA for self-assembly into nanorings. The replication efficacy of these replicons was evaluated both independently and in coupled assembly-replication reactions, with Qß chosen for its high amplification efficiency and template versatility. To mitigate the challenge of parasite RNA formation, which depletes essential replication components, a water-emulsion system was employed. The modular assembly of replicons into nanorings was further investigated, with a focus on system reproducibility and the effect of Mg2+ concentrations on nanoring stability. The final goal is to integrate self-replication with self-assembly, thereby creating a segmented, modular RNA genome for in vitro genetic information storage. This work addresses key challenges and advancements in developing synthetic self-replication systems for eventual genomic transplantation in E. coli.en
dc.language.isoende
dc.subjectRNAen
dc.subjectSelf-replicating RNAen
dc.subjectCell-freeen
dc.subjectpRNAen
dc.subject.ddc570-
dc.subject.ddc540-
dc.titleTowards the design and construction of self-replicating RNA nanostructuresen
dc.typeTextde
dc.contributor.refereeSummerer, Daniel-
dc.date.accepted2024-05-10-
dc.type.publicationtypePhDThesisde
dc.subject.rswkRNSde
dcterms.accessRightsopen access-
eldorado.secondarypublicationfalsede
Appears in Collections:Chemische Biologie

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