Yield improvement of exopolysaccharides by screening of the Lactobacillus acidophilus ATCC and optimization of the fermentation and extraction conditions
| dc.contributor.author | Liu, Qi | |
| dc.contributor.author | Huang, Xingjian | |
| dc.contributor.author | Yang, Dengxiang | |
| dc.contributor.author | Si, Tianlei | |
| dc.contributor.author | Pan, Siyi | |
| dc.contributor.author | Yang, Fang | |
| dc.date.accessioned | 2016-06-13T09:24:24Z | |
| dc.date.available | 2016-06-13T09:24:24Z | |
| dc.date.issued | 2016-02-12 | |
| dc.description.abstract | Exopolysacharides (EPS) produced by Lactobacillus acidophilus play an important role in food processing with its well-recognized antioxidant activity. In this study, a L. acidophilus mutant strain with high-yielding EPS (2.92±0.05 g/L) was screened by chemical mutation (0.2 % diethyl sulfate). Plackett-Burman (PB) design and response surface methodology (RSM) were applied to optimize the EPS fermentation parameters and central composite design (CCD) was used to optimize the EPS extraction parameters. A strain with high-yielding EPS was screened. It was revealed that three parameters (Tween 80, dipotassium hydrogen phosphate and trisodium citrate) had significant influence (P < 0.05) on the EPS yield. The optimal culture conditions for EPS production were: Tween 80 0.6 mL, dipotassium hydrogen phosphate 3.6 g and trisodium citrate 4.1 g (with culture volume of 1 L). In these conditions, the maximum EPS yield was 3.96±0.08 g/L. The optimal extraction conditions analyzed by CCD were: alcohol concentration 70 %, the ratio of material to liquid (M/L ratio) 1:3.6 and the extraction time 31 h. In these conditions, the maximum EPS extraction yield was 1.48±0.23 g/L. It was confirmed by the verification experiments that the EPS yield from L. acidophilus mutant strains reached 5.12±0.73 g/L under the optimized fermentation and extraction conditions, which was 3.8 times higher than that of the control (1.05±0.06 g/L). The results indicated that the strain screening with high-yielding EPS was successful and the optimized fermentation and extraction conditions significantly enhanced EPS yield. It was efficient and industrially promising. | en |
| dc.identifier.doi | 10.17179/excli2015-356 | |
| dc.identifier.issn | 1611-2156 | |
| dc.identifier.uri | http://hdl.handle.net/2003/35088 | |
| dc.identifier.uri | http://dx.doi.org/10.17877/DE290R-17136 | |
| dc.language.iso | en | |
| dc.relation.ispartofseries | EXCLI Journal;Vol. 15, 2016 | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0 | |
| dc.subject | Plackett-Burman design | en |
| dc.subject | central composite design | en |
| dc.subject | EPS | en |
| dc.subject | fermentation optimization | en |
| dc.subject | extraction optimization | en |
| dc.subject.ddc | 610 | |
| dc.title | Yield improvement of exopolysaccharides by screening of the Lactobacillus acidophilus ATCC and optimization of the fermentation and extraction conditions | en |
| dc.type | Text | |
| dc.type.publicationtype | article | |
| dcterms.accessRights | open access | |
| eldorado.dnb.zdberstkatid | 2132560-1 |