Senescent HUVECs-secreted exosomes trigger endothelial barrier dysfunction in young endothelial cells
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Date
2019-09-03
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Abstract
Accumulation of senescent endothelial cells can cause endothelium dysfunction which eventually leads to age-related vascular disorders. The senescent-associated secretory phenotype (SASP) cells
secrete a plethora of soluble factors that negatively influence the surrounding tissue microenvironment. The present study sought to investigate the effects of exosomes, which are nano-sized extracellular vesicl
es known for intercellular communications secreted by SASP cells on young
endothelial cells. Exosomes were isolated from the condition media of senescent human umbilical vein endothelial cells (HUVECs) and then confirmed by the detection of exosome specific CD63 and CD9 expressions, electron microscopy and acetylcholinesterase assay. The purified exosomes were used to treat young HUVECs. Exposure to exosomes repressed the expression of adherens junction proteins
including vascular endothelial (VE)-cadherin and beta-catenin, decreased cell growth kinetics and impaired endothelial migration potential of young endothelial cells. These findings suggest that senescent HUVECs-secreted exosomes could disrupt barrier integrity that underpins endothelial barrier dysfunction in healthy young endothelial cells.
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Keywords
Microvesicular, Extracellular vesicle, Senescent-associated secretory phenotype (SASP), Endothelial adherens junction proteins, Senescent HUVECs-secreted exosomes, Endothelial barrier dysfunction