Investigating the effect of visfatin on ERalpha phosphorylation (Ser118 and Ser167) and ERE-dependent transcriptional activity

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dc.contributor.authorZangooei, Mohammad
dc.contributor.authorNourbakhsh, Mitra
dc.contributor.authorGhahremani, Mohammad Hossein
dc.contributor.authorMeshkani, Reza
dc.contributor.authorKhedri, Azam
dc.contributor.authorShadboorestan, Amir
dc.contributor.authorShokri Afra, Hajar
dc.contributor.authorShahmohamadnejad, Shiva
dc.contributor.authorMirmiranpour, Hossein
dc.contributor.authorKhaghani, Shahnaz
dc.date.accessioned2018-10-22T06:51:11Z
dc.date.available2018-10-22T06:51:11Z
dc.date.issued2018-06-04
dc.description.abstractObesity is associated with higher postmenopausal breast cancer incidence. Visfatin level alteration is one of the mechanisms by which obesity promotes cancer. Ligand-independent activation of estrogen receptor alpha (ERα) is also associated with carcinogenesis. The activity of ERα is modulated through phosphorylation on multiple sites by a number of protein kinases. Here we investigated the effect of visfatin as a novel adipocytokine on the phosphorylation and activity of ERα in MCF-7 breast cancer cells. We showed that exogenous administration of visfatin significantly increased the phosphorylation of ERα at serine 118 (Ser118) and 167 (Ser167) residues. Visfatininduced Ser118 phosphorylation was diminished after treatment of cells with U0126 (MEK1/2 inhibitor). Furthermore, our results showed that visfatin-induced Ser167 phosphorylation is mediated through both MAPK and PI3K/Akt signaling pathways. Inhibition of the enzymatic activity of visfatin by FK866 had no effect on phosphorylation of ERα. We also showed that visfatin enhanced the estrogen response element (ERE)-dependent activity of ER in the presence of 17-β estradiol (E2). Additional study on T47D cells showed that visfatin also increased Ser118 and Ser167 phosphorylation of ERα and enhanced ERE-dependent activity in the presence of E2 in these cells.en
dc.identifier.citationEXCLI Journal 2018;17:516-525de
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/37204
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-19199
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal;Vol. 17 2018
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBreast canceren
dc.subjectEstrogen receptoren
dc.subjectSerine 118 phosphorylationen
dc.subjectSerine 167 phosphorylationen
dc.subjectVisfatinen
dc.subject.ddc610
dc.titleInvestigating the effect of visfatin on ERalpha phosphorylation (Ser118 and Ser167) and ERE-dependent transcriptional activityen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.secondarypublicationtrue

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