Knockdown of CSNK2β suppresses MDA-MB231 cell growth, induces apoptosis, inhibits migration and invasion
| dc.contributor.author | Karna, Shibendra Kumar Lal | |
| dc.contributor.author | Lone, Bilal Ahmad | |
| dc.contributor.author | Ahmad, Faiz | |
| dc.contributor.author | Shahi, Nerina | |
| dc.contributor.author | Pokharel, Yuba Raj | |
| dc.date.accessioned | 2020-12-14T14:41:05Z | |
| dc.date.available | 2020-12-14T14:41:05Z | |
| dc.date.issued | 2020-09-07 | |
| dc.description.abstract | Breast cancer is the most common cancer among women worldwide. Among different types of breast cancer known, treatment of triple-negative breast cancer is a major challenge because of its aggressiveness and poor prognosis; thus, identification of specific drivers is required for targeted therapies of breast cancer malignancy. Protein Casein Kinase (CSNK) is a serine/threonine kinase that exists as a tetrameric complex consisting of two catalytic (α and /or α') and two regulatory β subunits. CSNK2β can also function independently without catalytic subunits and exist as a distinct population in cells. This study aims to elucidate the role of Casein Kinase 2β (CSNK2β) gene in cell proliferation, cell cycle, migration and apoptosis of triple-negative breast cancer MDA-MB-231 cells. The silencing of CSNK2β in MDA-MB-231 cells resulted in decreased cell viability and colony formation. Cell cycle analysis showed a significant arrest of cells in G2M phase. Hoechst and CM-H2DCFDA staining showed nuclear condensation and augmented intracellular reactive oxygen species (ROS) production. Furthermore, silencing of CSNK2β in MDA-MB-231 cells modulated the apoptotic machinery- BAX, Bcl-xL, and caspase 3; autophagy machinery-Beclin-1 and LC3-1; and inhibited the vital markers (p-ERK, c-Myc, NF-κB, E2F1, PCNA, p38-α) associated with cell proliferation and DNA replication pathways. In addition, knockdown of CSNK2β also affected the migration potential of MDA-MB-231, as observed in the wound healing and transwell migration assays. Altogether, the study suggests that CSNK2β silencing may offer future therapeutic target in triple-negative breast cancer. | en |
| dc.identifier.citation | Karna, S. K. L., Lone, B. A., Ahmad, F., Shahi, N., & Pokharel, Y. R. (2020). Knockdown of CSNK2β suppresses MDA-MB231 cell growth, induces apoptosis, inhibits migration and invasion. EXCLI Journal, 19, 1211-1226. https://doi.org/10.17179/excli2020-2363 | de |
| dc.identifier.issn | 1611-2156 | |
| dc.identifier.uri | http://hdl.handle.net/2003/39889 | |
| dc.identifier.uri | http://dx.doi.org/10.17877/DE290R-21779 | |
| dc.language.iso | en | |
| dc.publisher | IfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmund | de |
| dc.relation.ispartofseries | EXCLI Journal;Vol. 19. 2020, pp.1211-1226 | |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | CSNK2β | en |
| dc.subject | Breast cancer | en |
| dc.subject | Apoptosis | en |
| dc.subject | Migration | en |
| dc.subject.ddc | 610 | |
| dc.title | Knockdown of CSNK2β suppresses MDA-MB231 cell growth, induces apoptosis, inhibits migration and invasion | en |
| dc.type | Text | |
| dc.type.publicationtype | article | |
| dcterms.accessRights | open access | |
| eldorado.dnb.zdberstkatid | 2132560-1 | |
| eldorado.identifier.url | https://www.excli.de/index.php/excli/article/view/2363 | |
| eldorado.secondarypublication | true |