Induction and repression effects on CYP and transporter protein abundance by azole mixture uptake in rat liver

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dc.contributor.authorHammer, Helen
dc.contributor.authorSchmidt, Flavia
dc.contributor.authorHeise, Tanja
dc.contributor.authorKnebel, Constanze
dc.contributor.authorDabrowski, Alexander
dc.contributor.authorPlanatscher, Hannes
dc.contributor.authorKneuer, Carsten
dc.contributor.authorMarx-Stoelting, Philip
dc.contributor.authorPötz, Oliver
dc.date.accessioned2020-12-11T13:30:54Z
dc.date.available2020-12-11T13:30:54Z
dc.date.issued2020-06-26
dc.description.abstractDetection of mixture effects is a major challenge in current experimental and regulatory toxicology. Robust markers are needed that are easy to quantify and responsive to chemical stressors in a broad dose range. Several hepatic enzymes and proteins related to drug metabolism like cytochrome-P-450 (CYP) enzymes and transporters have been shown to be responsive to pesticide active substances in a broad dose range and are therefore good candidates to be used as markers for mixture toxicity. Even though they can be well quantified at the mRNA level, quantification on the protein level is challenging because most of these proteins are membrane bound. Here we report the development of mass spectrometry-based assays using triple-x-proteomics (TXP) antibodies in combination with targeted selected ion monitoring (tSIM) to quantify changes of protein levels due to exposure to mixtures of pesticide active substances. Our results indicate that changes on the protein level of CYP1A1, ABCB2, ABCC3 are in line with observations on the mRNA and enzyme activity level and are indicative of mixture effects. Therefore, the tests are promising to reveal effects by chemical mixture effects in toxicological studies in rats.en
dc.identifier.citationHammer, H., Schmidt, F., Heise, T., Knebel, C., Dabrowski, A., Planatscher, H., Kneuer, C., Marx-Stoelting, P., & Pötz, O. (2020). Induction and repression effects on CYP and transporter protein abundance by azole mixture uptake in rat liver. EXCLI Journal, 19, 904-916. https://doi.org/10.17179/excli2020-2311en
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/39877
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-21768
dc.language.isoen
dc.publisherIfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmunden
dc.relation.ispartofseriesEXCLI Journal;Vol. 19 2020
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectABC transporteren
dc.subjectAzole fungicidesen
dc.subjectCytochrome P450en
dc.subjectImmunoaffinity-based mass spectrometryen
dc.subjectLiver toxicityen
dc.subjectPesticidesen
dc.subjectPesticide mixturesen
dc.subjectSLC transporteren
dc.subject.ddc610
dc.titleInduction and repression effects on CYP and transporter protein abundance by azole mixture uptake in rat liveren
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.identifier.urlhttps://www.excli.de/index.php/excli/article/view/2311
eldorado.secondarypublicationtrue

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