Neuroprotective effect of Azadirachta indica standardized extract in partial sciatic nerve injury in rats

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dc.contributor.authorKandhare, Amit D.
dc.contributor.authorMukherjee, Anwesha A.
dc.contributor.authorBodhankar, Subhash L.
dc.date.accessioned2017-10-26T12:04:56Z
dc.date.available2017-10-26T12:04:56Z
dc.date.issued2017-04-18
dc.description.abstractChronic neuropathic pain is a common and widely recognized pain syndrome for patients and difficult to manage for physicians. Azadirachta indica (AI) possesses analgesic, anti-inflammatory, and antioxidant properties. To evaluate the neuroprotective effect of AI standardized extract in an animal model of peripheral neuropathy induced by partial sciatic nerve ligation (PSNL). PSNL was induced in male Wistar rats (180-200 g) with tight ligation of the nerve. Rats received treatment with either vehicle i.e. distilled water (PSNL control), Pyridoxine (100 mg/kg, p.o.) or AI (100, 200 and 400 mg/kg, p.o.) for 28 days. Various behavioral parameters, biochemical, molecular and histological parameters were evaluated. PSNL resulted in a significant decrease (p < 0.05) in allodynia, hy- peralgesia, motor coordination and motor nerve conduction velocity (MNCV) whereas chronic treatment with AI (200 and 400 mg/kg) significantly attenuated (p < 0.05) these behavioral changes. Enhanced activity of oxidative- nitrosative stress, inflammatory mediators (TNF-α, IL-1β, and NF-κB) as well as mRNA expression of Bax, Caspase-3, and iNOs were significantly attenuated (p < 0.05) by AI treatment. It also significantly increased (p < 0.05) peripheral blood oxygen content and Bcl-2 mRNA expression. The flow cytometric analysis revealed that AI (200 and 400 mg/kg) treatment significantly attenuated neural apoptosis and reactive oxygen species levels. PSNL induced histological aberrations were also decreased by AI treatment. Azadirachta indica exerts its neuro- protection against PSNL induced neuropathic pain via inhibition of oxidative-nitrosative stress, the release of pro-inflammatory cytokines and apoptosis to improve MNCV (graphical abstract, Figure 1).en
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/36147
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-18163
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal;Vol. 16 2017
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAzadirachta indicaen
dc.subjectpartial sciatic nerve ligationen
dc.subjectneuropathic painen
dc.subjectNF-κBen
dc.subjectBAXen
dc.subjectcaspase-3en
dc.subjectapoptosisen
dc.subjectflow cytometric analysisen
dc.subjectmotor nerve conduction velocityen
dc.subject.ddc610
dc.titleNeuroprotective effect of Azadirachta indica standardized extract in partial sciatic nerve injury in ratsen
dc.title.alternativeevidence from anti-inflammatory, antioxidant and anti-apoptotic studiesen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.secondarypublicationtrue

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