MiR-429 suppresses the progression and metastasis of osteosarcoma by targeting ZEB1
| dc.contributor.author | Deng, Yi | |
| dc.contributor.author | Luan, Fujun | |
| dc.contributor.author | Zeng, Li | |
| dc.contributor.author | Zhang, Yanjun | |
| dc.contributor.author | Ma, Kunlong | |
| dc.date.accessioned | 2017-10-26T13:25:26Z | |
| dc.date.available | 2017-10-26T13:25:26Z | |
| dc.date.issued | 2017-05-05 | |
| dc.description.abstract | MiR-429 functions as a tumor suppressor and has been observed in multiple types of cancer, but the effects and mechanisms of miR-429 in osteosarcoma are poorly understood. This study is performed to evaluate the functions of miR-429 in the progression of osteosarcoma. Firstly, the miR-429 expression in osteosarcoma tissues and oste- osarcoma cells was detected using real time PCR, and the relationship between miR-429 expression and overall survival of osteosarcoma was analyzed. Secondly, the effects of miR-429 on the migration, invasion, proliferation and apoptosis of osteosarcoma cells were evaluated using transwell assay, wound-healing assay, CCK-8 assay and flow cytometry, respectively. Proteins related to epithelial-mesenchymal transition (EMT), E-cadherin, Vimentin, N-cadherin and Snail, were also detected using Western blot. Finally, the target gene of miR-429 in osteosarcoma was predicted and verified using dual luciferase assay and the expression correlation between them was analyzed using Pearson’s correlation. MiR-429 was down-regulated in osteosarcoma tissues and osteosarcoma cells; the expression level of miR-429 was associated with the prognosis of osteosarcoma. High level of miR-429 in osteo- sarcoma cells significantly suppressed the migration, invasion and proliferation of cells but induced cells apopto- sis. Furthermore, high level of miR-429 in osteosarcoma cells obviously increased the expression of E-cadherin protein but decreased the expression of Vimentin, N-Cadherin and Snail proteins. EMT inducer ZEB1 was the target gene of miR-429 and the expression of ZEB1 was negatively related to the miR-429 expression in osteosar- coma. In conclusion, miR-429 may functions as a tumor suppressor and be down-regulated in osteosarcoma. MiR- 429 may suppress the progression and metastasis of osteosarcoma by down-regulating the ZEB1 expression. | en |
| dc.identifier.issn | 1611-2156 | |
| dc.identifier.uri | http://hdl.handle.net/2003/36151 | |
| dc.identifier.uri | http://dx.doi.org/10.17877/DE290R-18167 | |
| dc.language.iso | en | |
| dc.relation.ispartofseries | EXCLI Journal;Vol. 16 2017 | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | miR-429 | en |
| dc.subject | osteosarcoma | en |
| dc.subject | progression | en |
| dc.subject | EMT | en |
| dc.subject | ZEB1 | en |
| dc.subject.ddc | 610 | |
| dc.title | MiR-429 suppresses the progression and metastasis of osteosarcoma by targeting ZEB1 | en |
| dc.type | Text | |
| dc.type.publicationtype | article | |
| dcterms.accessRights | open access | |
| eldorado.dnb.zdberstkatid | 2132560-1 | |
| eldorado.secondarypublication | true |