A nanoporous hydrogel-based model to study chemokine gradient-driven angiogenesis under luminal flow
| dc.contributor.author | Mote, Nidhi | |
| dc.contributor.author | Kubik, Sarah | |
| dc.contributor.author | Polacheck, William J. | |
| dc.contributor.author | Baker, Brendon M. | |
| dc.contributor.author | Trappmann, Britta | |
| dc.date.accessioned | 2025-09-24T10:27:54Z | |
| dc.date.available | 2025-09-24T10:27:54Z | |
| dc.date.issued | 2024-09-11 | |
| dc.description.abstract | The growth of new blood vessels through angiogenesis is a highly coordinated process, which is initiated by chemokine gradients that activate endothelial cells within a perfused parent vessel to sprout into the surrounding 3D tissue matrix. While both biochemical signals from pro-angiogenic factors, as well as mechanical cues originating from luminal fluid flow that exerts shear stress on the vessel wall, have individually been identified as major regulators of endothelial cell sprouting, it remains unclear whether and how both types of cues synergize. To fill this knowledge gap, here, we created a 3D biomimetic model of chemokine gradient-driven angiogenic sprouting, in which a micromolded tube inside a hydrogel matrix is seeded with endothelial cells and connected to a perfusion system to control fluid flow rates and resulting shear forces on the vessel wall. To allow for the formation of chemokine gradients despite the presence of luminal flow, a nanoporous synthetic hydrogel that supports angiogenesis but limits the interstitial flow proved crucial. Using this system, we find that luminal flow and resulting shear stress is a major regulator of the speed and morphogenesis of angiogenic sprouting, whose action is mediated through changes in vascular permeability. | en |
| dc.identifier.uri | http://hdl.handle.net/2003/43958 | |
| dc.language.iso | en | |
| dc.relation.ispartofseries | Lab on a chip; 24(20) | |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/ | |
| dc.subject.ddc | 540 | |
| dc.title | A nanoporous hydrogel-based model to study chemokine gradient-driven angiogenesis under luminal flow | en |
| dc.type | Text | |
| dc.type.publicationtype | Article | |
| dcterms.accessRights | open access | |
| eldorado.dnb.deposit | true | |
| eldorado.doi.register | false | |
| eldorado.secondarypublication | true | |
| eldorado.secondarypublication.primarycitation | Mote, N., Kubik, S., Polacheck, W. J., Baker, B. M., & Trappmann, B. (2024). A nanoporous hydrogel-based model to study chemokine gradient-driven angiogenesis under luminal flow. Lab on a Chip, 24(20), 4892–4906. https://doi.org/10.1039/d4lc00460d | |
| eldorado.secondarypublication.primaryidentifier | https://doi.org/10.1039/D4LC00460D |