Trans-1,3-diphenyl-2,3-epoxypropan-1-one, a chalcone derivative, induces apoptosis via ROS-mediated down-regulation of Bcl-xL in human leukemia HL-60 cells

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dc.contributor.authorKo, Eun-Yi
dc.contributor.authorLee, Seung-Hong
dc.contributor.authorKo, Ji-Yeon
dc.contributor.authorMoon, Jeong Yong
dc.contributor.authorYoon, Weon-Jong
dc.contributor.authorAhn, Ginnae
dc.contributor.authorRoh, Seong Woon
dc.contributor.authorCho, Kichul
dc.contributor.authorJeon, You-Jin
dc.contributor.authorKim, Daekyung
dc.contributor.authorKim, Kil-Nam
dc.date.accessioned2016-06-03T16:18:41Z
dc.date.available2016-06-03T16:18:41Z
dc.date.issued2015-08-03
dc.description.abstractThe anticancer effects of trans-1,3-diphenyl-2,3-epoxypropan-1-one (DPEP), a chalcone derivative, were investigated in human leukemia HL-60 cells. Treatment of HL-60 cells with various concentration of DPEP resulted in a sequence of events characteristic of apoptosis, including loss of cell viability, morphological changes, and increased sub-G1 DNA content. We demonstrated that DPEP elevates reactive oxygen species (ROS) levels in HL-60 cells, and that the ROS scavenger N-acetylcysteine (NAC) could block DPEP-induced ROS generation and apoptosis. Western blot analysis revealed that DPEP inhibits Bcl-xL expression, leading to caspase-3 activation and poly-ADP-ribose polymerase (PARP) cleavage, thereby inducing apoptosis. However, NAC pretreatment significantly inhibited the activation of caspase-3 and PARP cleavage and reduced Bcl-xL levels. These findings provide the first evidence that DPEP may inhibit the growth of HL-60 cells and induce apoptosis through a ROS-mediated Bcl-xL pathway.en
dc.identifier.doi10.17179/excli2015-373
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/35025
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-17073
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal;Vol. 14, 2015
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectanticanceren
dc.subjecttrans-1,3-diphenyl-2,3-epoxypropan-1-one (DPEP)en
dc.subjectapoptosisen
dc.subjectreactive oxygen species (ROS)en
dc.subjectBcl-xLen
dc.subject.ddc610
dc.titleTrans-1,3-diphenyl-2,3-epoxypropan-1-one, a chalcone derivative, induces apoptosis via ROS-mediated down-regulation of Bcl-xL in human leukemia HL-60 cellsen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1

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