Bioinformatics and experimental studies of anti-leukemic activity from 6-gingerol demonstrate its role in p53 mediated apoptosis pathway

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dc.contributor.authorChatupheeraphat, Chawalit
dc.contributor.authorNantasenamat, Chanin
dc.contributor.authorDeesrisak, Kamolchanok
dc.contributor.authorRoytrakul, Sittiruk
dc.contributor.authorAnurathapan, Usanarat
dc.contributor.authorTanyong, Dalina
dc.date.accessioned2020-12-11T13:02:47Z
dc.date.available2020-12-11T13:02:47Z
dc.date.issued2020-05-06
dc.description.abstract6-gingerol is a traditional medicine that possesses anti-cancer activity against several types of cancer. However, the mechanism of action still remains unclear. Therefore, this study explored the effects of 6-gingerol on anti-leukemic mechanisms in NB4, MOLT4, and Raji leukemic cell. Results indicated that 6-gingerol inhibited cell proliferation and induced cell apoptosis in these 3 cell lines. Moreover, 6-gingerol was shown to increase the mRNA expression of the caspase family thereby suggesting that 6-gingerol induced apoptosis through the caspase-dependent pathway. To explore the signaling pathway regulating 6-gingerol induced apoptosis, we utilized and integrated the network pharmacology approach together with experimental investigations. Targets of 6-gingerol were identified from ChEMBL and STITCH databases, which were used for constructing the protein-protein interaction (PPI) network. Results from the PPI network indicated that p53 was a key regulator. Moreover, it was found that 6-gingerol could increase the levels of p53 mRNA in all leukemic cell lines. Thus, 6-gingerol has shown to have anti-cancer activity. In addition, p53, BAX and BCL2 could be involved in the apoptosis pathway of these leukemic cells. This study is anticipated to be useful for the development of 6-gingerol as an anti-leukemic drug in the future.en
dc.identifier.citationChatupheeraphat, C., Nantasenamat, C., Deesrisak, K., Roytrakul, S., Anurathapan, U., & Tanyong, D. (2020). Bioinformatics and experimental studies of anti-leukemic activity from 6-gingerol demonstrate its role in p53 mediated apoptosis pathway. EXCLI Journal, 19, 582-595. https://doi.org/10.17179/excli2019-2008en
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/39874
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-21765
dc.language.isoen
dc.publisherIfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmunden
dc.relation.ispartofseriesEXCLI Journal;Vol. 19 2020
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject6-gingerolen
dc.subjectLeukemiaen
dc.subjectApoptosisen
dc.subjectNetwork pharmacologyen
dc.subjectp53en
dc.subjectBioinformaticsen
dc.subject.ddc610
dc.titleBioinformatics and experimental studies of anti-leukemic activity from 6-gingerol demonstrate its role in p53 mediated apoptosis pathwayen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.identifier.urlhttps://www.excli.de/index.php/excli/article/view/1805
eldorado.secondarypublicationtrue

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