Evaluation of microsatellite instability in tumor and tumor marginal samples of sporadic colorectal cancer using mononucleotide markers
Loading...
Date
2018-09-24
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Microsatellite instability (MSI) is a unique molecular alteration that is due to a defective DNA mismatch repair
(MMR) system. Approximately, 15-20 % of sporadic colorectal cancers (CRC) display MSI. Determination of
MSI status in CRC has prognostic and predictive implications. Additionally, detecting MSI is used diagnostically
for tumor detection and classification. The present study analyzed a panel of five mononucleotide markers, BAT-
25, BAT-26, NR-21, NR-22 and NR-27, amplified in a single multiplex PCR reaction to evaluate MSI status in
CRC patients. Genomic DNA from 50 CRC and paired adjacent normal tissues was used for PCR-based MSI
analysis. Our finding showed microsatellite instability in 36 % of specimens. Instability with differences in allele
lengths was observed in the tumoral DNA compared to the tumor-free margin DNA sample. The frequency of
instability in NR-21, BAT-26 and BAT-25 markers were more than others; their frequency were 35.48 %, 29.03 %,
and 22.58 %, respectively. In conclusion, the NR-21, BAT-26, and BAT-25 were the most useful markers for
discriminating cancer tissue from normal, therefore these markers have demonstrated promising potential for determining
MSI status in patients with sporadic colorectal cancer.
Description
Table of contents
Keywords
CRC, MSI, DNA MMR system