Effect of elaidic acid on ABCA1 expression in RAW 264.7 cells.
Loading...
Date
2018-08-28
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
In recent years, Trans Fatty Acids have shown a strong correlation with cardiovascular disease. However, the
mechanisms explaining their atherogenicity are still unclear. ABCA1, which is involved in the reverse cholesterol
transport pathway, has been considered as a new therapeutic target for cardiovascular disease. In vitro studies of
the effects of PPAR-γ on lipid homeostasis in macrophage cells suggested a role for PPAR-γ in the regulation of
ABCA1-dependent cholesterol efflux to apoA-I pathway. Thus, in this study we examined the effect of elaidic
acid (EA) as the most abundant TFA on expression of ABCA1 and PPAR-γ in RAW 264.7 mouse macrophage
cell line. Accordingly, after determining appropriate concentrations of EA using MTT, RAW 264.7 cells were
treated with different concentrations of EA, and at the end, gene expression was assayed by Real-Time PCR. Our
results shown that the expression of ABCA1 decreased in the treated group in comparison with the control group
by 1.7, 2.3, and 5.1 fold, after 12 h treatment for 0.5, 1, and 2 mM EA concentration respectively. In addition, after
24 h treatment with EA, the rate of decreasing ABCA1 expression was 2.1, 2.6, 5.7 fold, respectively (P < 0.01).
However, EA had no significant effect on PPAR-γ mRNA expression. Therefore, it could be concluded that the
atherogenic effect of EA may be mediated by reducing ABCA1 expression in RAW 264.7 cells; however, this
reduction has not mediated through altering PPAR-γ expression.
Description
Table of contents
Keywords
Trans fatty acid, Atherosclerosis, ABCA1, Gene expression