Microbiological and virulence aspects of Rhodotorula mucilaginosa

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dc.contributor.authorJarros, Isabele Carrilho
dc.contributor.authorVeiga, Flávia Franco
dc.contributor.authorCorrêa, Jakeline Luiz
dc.contributor.authorBarros, Isabella Letícia Esteves
dc.contributor.authorGadelha, Marina Cristina
dc.contributor.authorVoidaleski, Morgana F.
dc.contributor.authorPieralisi, Neli
dc.contributor.authorPedroso, Raissa Bocchi
dc.contributor.authorVicente, Vânia A.
dc.contributor.authorNegri, Melyssa
dc.contributor.authorSvidzinski, Terezinha Inez Estivalet
dc.date.accessioned2020-12-11T13:14:58Z
dc.date.available2020-12-11T13:14:58Z
dc.date.issued2020-05-27
dc.description.abstractWe aimed to characterize microbiologically clinical isolates of R. mucilaginosa isolated from colonization of a patient with chronic renal disease (CKD), as well as to evaluate their phylogeny, antifungal susceptibility, virulence, and pathogenicity in order to infer the potential to become a possible infective agent. For this study, two isolates of R. mucilaginosa from oral colonization of a CKD patient were isolated, identified and characterized by classical (genotypic and phenotypic) methods. Susceptibility to conventional antifungals was evaluated, followed by biofilm production, measured by different techniques (total biomass, metabolic activity, colony forming units and extracellular matrix quantification). Finally, the pathogenicity of yeast was evaluated by infection of Tenebrio molitor larvae. All isolates were resistant to azole and sensitive to polyenes and they were able to adhere and form biofilm on the abiotic surface of polystyrene. In general, similar profiles among isolates were observed over the observed periods (2, 24, 48 and 72 hours). Regarding extracellular matrix components of biofilms at different maturation ages, R. mucilaginosa was able to produce eDNA, eRNA, proteins, and polysaccharides that varied according to time and the strain. The death curve in vivo model showed a large reduction in the survival percentage of the larvae was observed in the first 24 hours, with only 40 % survival at the end of the evaluation. We infer that colonization of chronic renal patients by R. mucilaginosa offers a high risk of serious infection. And also emphasize that the correct identification of yeast is the main means for an efficient treatment.en
dc.identifier.citationJarros, I. C., Veiga, F. F., Corrêa, J. L., Barros, I. L. E., Gadelha, M. C., Voidaleski, M. F., Pieralisi, N., Pedroso, R. B., Vicente, V. A., Negri, M., & Svidzinski, T. I. E. (2020). Microbiological and virulence aspects of Rhodotorula mucilaginosa. EXCLI Journal, 19, 687-704. https://doi.org/10.17179/excli2019-1672en
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/39875
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-21766
dc.language.isoen
dc.publisherIfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmunden
dc.relation.ispartofseriesEXCLI Journal;Vol. 19 2020
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectRhodotorula mucilaginosaen
dc.subjectCharacterization of biofilmen
dc.subjectTenebrio molitoren
dc.subject.ddc610
dc.titleMicrobiological and virulence aspects of Rhodotorula mucilaginosaen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.identifier.urlhttps://www.excli.de/index.php/excli/article/view/1672
eldorado.secondarypublicationtrue

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