MicroRNA-9-5p functions as a tumor suppressor in prostate cancer via targeting UTRN

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dc.contributor.authorGuo, Zhenyu
dc.contributor.authorHuang, Shuchang
dc.contributor.authorZhou, Yihong
dc.contributor.authorCheng, Wenjie
dc.contributor.authorChu, Xi
dc.contributor.authorZheng, Xiaoqing
dc.contributor.authorZheng, Hao
dc.date.accessioned2020-05-19T07:54:47Z
dc.date.available2020-05-19T07:54:47Z
dc.date.issued2019-11-07
dc.description.abstractAccumulating evidence indicates that miR-9-5p plays an important role in several diseases, especially tumor progression. In this study, we investigated the clinical significance and biological function of miR-9-5p in prostate cancer (PCa). Using quantitative real time PCR (qRT-PCR) analysis, we found miR-9-5p level was significantly down-regulated in PCa tissues and cell lines. The decreased miR-9-5p expression was associated with tumor size, preoperative PSA, Gleason score and lymph node metastasis. Kaplan-Meier survival analysis showed patients with low level of miR-9-5p had significantly decreased rates of overall survival (OS). Multivariate analyses showed that miR-9-5p was an independent predictor of PCa patients’ prognosis. Through CCK-8 and Transwell assays, miR-9-5p overexpression by miR-9-5p mimics transfection was demonstrated to suppress the proliferation, migration and invasion of PCa cells. Mechanistically, luciferase reporter assay, qRT-PCR and Western blot demonstrated that Utrophin (UTRN) is a direct target of miR-9-5p in PCa cells. The status of UTRN protein in PCa tissues was much higher than that in adjacent tissues by immunohistochemical staining and its mRNA levels were inversely correlated with miR-9-5p in PCa tissues. Importantly, UTRN knockdown by siUTRN imitated the suppressive effects of miR-9-5p on cell proliferation, migration and invasion in PCa. In summary, miR-9-5p might novel prognostic biomarker in and targeting UTRN by miR-9-5p could be potential therapeutic candidates for PCa.en
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/39113
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-21031
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal;Vol. 18 2019
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectProstate canceren
dc.subjectmiR-9-5Pen
dc.subjectUTRNen
dc.subjectPrognosisen
dc.subjectCell proliferationen
dc.subjectMigrationen
dc.subject.ddc610
dc.titleMicroRNA-9-5p functions as a tumor suppressor in prostate cancer via targeting UTRNen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.secondarypublicationtrue

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