Crocetin promotes angiogenesis in human endothelial cells through PI3K-Akt-eNOS signaling pathway

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dc.contributor.authorNasirzadeh, Mahdieh
dc.contributor.authorRasmi, Yousef
dc.contributor.authorRahbarghazi, Reza
dc.contributor.authorKheradmand, Fatemeh
dc.contributor.authorKarimipour, Mojtaba
dc.contributor.authorAramwit, Pornanong
dc.contributor.authorAstinfeshan, Maryam
dc.contributor.authorGholinejad, Zafar
dc.contributor.authorDaeihasani, Behrokh
dc.contributor.authorSaboory, Ehsan
dc.contributor.authorShirpoor, Alireza
dc.contributor.authorRezabakhsh, Aysa
dc.contributor.authorZolali, Elmira
dc.contributor.authorKhalaji, Naser
dc.date.accessioned2020-03-09T12:12:49Z
dc.date.available2020-03-09T12:12:49Z
dc.date.issued2019-10-21
dc.description.abstractPrevious studies proved the pro-angiogenic effect of Crocetin, a natural carotenoid dicarboxylic acid, in both in vivo and in vitro models. However, the exact mechanism of Crocetin action has not completely been elucidated yet. The current experiment was designed to find the activity of PI3K-Akt-eNOS axis after the treatment of endothelial cells with Crocetin in vitro. Human Umbilical Vein Endothelial Cells (HUVECs) were incubated with various concentrations of Crocetin (1, 5, 25, 50, and 100 μM) over a period of 72 h. Crocetin significantly increased HUVECs viability after 72 h as compared with the control group. We also found that Crocetin promoted the formation of the capillary-like structure compared to the control (p<0.05). Moreover, an improved migration rate and increased MMP-9 activity were observed in HUVECs that received 50 μM Crocetin (p<0.05). Crocetin enhanced the uptake of Ac-LDL which is correlated with increased lipid metabolism. Based on the data from thecurrent experiment, protein level of VEGFR-1, -2 and p-Akt/Akt, p-eNOS/eNOS ratios were increased 72 h after the treatment of HUVECs with Crocetin (p<0.05). In contrast, the transcription level of VEGF was reduced in Crocetin-treated cells. These data demonstrated that Crocetin promotes HUVECs angiogenesis potential by the modulation of VEGF signaling pathway and increased cell viability. The PI3K/Akt/eNOS axis is required for a Crocetin-associated activity in endothelial cells.en
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/39059
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-20978
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal;Vol. 18 2019
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCrocetinen
dc.subjectHuman endothelial cellsen
dc.subjectAngiogenesisen
dc.subjectTube formationen
dc.subjectMigrationen
dc.subjectVEGFR-2-Akt-eNOS signalingen
dc.subject.ddc610
dc.titleCrocetin promotes angiogenesis in human endothelial cells through PI3K-Akt-eNOS signaling pathwayen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.secondarypublicationtrue

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