Engineered TALE repeats for enhanced imaging-based analysisof cellular 5-Methylcytosine

Authors:	Muñoz‐López, Alvaro Jung, Anne Buchmuller, Benjamin Wolffgramm, Jan Maurer, Sara Witte, Anna Summerer, Daniel
Title:	Engineered TALE repeats for enhanced imaging-based analysisof cellular 5-Methylcytosine
Language (ISO):	en
Abstract:	Two‐TALE test: Engineered transcription‐activator‐like effector (TALE) repeats can be used to design programmable receptors for improved in situ imaging‐based analysis of 5 mC in user‐defined DNA sequences. Pairs of natural 5 mC‐discriminating and engineered 5 mC‐binding TALEs allow nucleotide resolution analysis of 5 mC with response of both TALEs. This provides an impulse for the design of programmable imaging probes for studying 5 mC functions in chromatin regulation with increased dynamic range.
Subject Headings:	DNA methylation Epigenetics Imaging probes Membrane-less organelles
URI:	http://hdl.handle.net/2003/40205 http://dx.doi.org/10.17877/DE290R-22078
Issue Date:	2020-09-20
Rights link:	https://creativecommons.org/licenses/by/4.0/
Appears in Collections:	Chemische Biologie

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