Authors: | Muñoz‐López, Alvaro Jung, Anne Buchmuller, Benjamin Wolffgramm, Jan Maurer, Sara Witte, Anna Summerer, Daniel |
Title: | Engineered TALE repeats for enhanced imaging-based analysisof cellular 5-Methylcytosine |
Language (ISO): | en |
Abstract: | Two‐TALE test: Engineered transcription‐activator‐like effector (TALE) repeats can be used to design programmable receptors for improved in situ imaging‐based analysis of 5 mC in user‐defined DNA sequences. Pairs of natural 5 mC‐discriminating and engineered 5 mC‐binding TALEs allow nucleotide resolution analysis of 5 mC with response of both TALEs. This provides an impulse for the design of programmable imaging probes for studying 5 mC functions in chromatin regulation with increased dynamic range. |
Subject Headings: | DNA methylation Epigenetics Imaging probes Membrane-less organelles |
URI: | http://hdl.handle.net/2003/40205 http://dx.doi.org/10.17877/DE290R-22078 |
Issue Date: | 2020-09-20 |
Rights link: | https://creativecommons.org/licenses/by/4.0/ |
Appears in Collections: | Chemische Biologie |
Files in This Item:
File | Description | Size | Format | |
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cbic.202000563.pdf | 1.15 MB | Adobe PDF | View/Open |
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