Authors: Neumann, Heinz
Neumann-Staubitz, Petra
Witte, Anna
Summerer, Daniel
Title: Epigenetic chromatin modification by amber suppression technology
Language (ISO): en
Abstract: The genetic incorporation of unnatural amino acids (UAAs) into proteins by amber suppression technology provides unique avenues to study protein structure, function and interactions both in vitro and in living cells and organisms. This approach has been particularly useful for studying mechanisms of epigenetic chromatin regulation, since these extensively involve dynamic changes in structure, complex formation and posttranslational modifications that are difficult to access by traditional approaches. Here, we review recent achievements in this field, emphasizing UAAs that help to unravel protein-protein interactions in cells by photo-crosslinking or that allow the biosynthesis of proteins with defined posttranslational modifications for studying their function and turnover in vitro and in cells.
Subject Headings: Genetic Code Expansion
Unnatural Amino Acids
Post-translational modifications
Bioorthogonal Chemistry
Issue Date: 2018-02-22
Appears in Collections:Lehrstuhl für Chemische Biologie der Nukleinsäuren

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