Preventive effect of N-acetylcysteine in a mouse model of steroid resistant acute exacerbation of asthma
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Date
2013-05-23
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Abstract
Oxidative stress appears to have an important role in glucocorticoid insensitivity, as a crucial problem in asthma therapy. We studied the preventive effect of antioxidant N-acetylcysteine
(NAC) on the airway hyper-responsiveness (AHR) and the accumulation of inflammatory
cells in the airways in an animal model of steroid resistant acute exacerbation of asthma. Systemically sensitized Balb/C mice were exposed to Ovalbumin aerosol on days 13, 14, 15 and 16, followed by intratracheal lipopolysaccharide (LPS) to induce acute exacerbation. NAC (intraperitoneal, 320 mg/kg 30 min before and 12 hours after each challenge) reduced hyperresponsiveness
with/out dexamethasone. LPS application caused neutrophilia in bronchoalveolar
lavage fluid (BALF) and eosinophil count was higher than respective control in BALF as
well as neutrophils after dexamethasone treatment. NAC significantly decreased neutrophil and eosinophil count in BALF as well as inflammatory cytokines (IL-13 and IL-5).We concluded that addition of NAC to asthma therapy has beneficial preventive effects in an animal model of steroid resistant acute exacerbation of asthma.
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Keywords
acute exacerbation, eosinophil, N-acetylcysteine, neutrophil, steroid resistant asthma