MicroRNA-494 induces breast cancer cell apoptosis and reduces cell viability by inhibition of nicotinamide phosphoribosyltransferase expression and activity
| dc.contributor.author | Ghorbanhosseini, Seyedeh Sara | |
| dc.contributor.author | Nourbakhsh, Mitra | |
| dc.contributor.author | Zangooei, Mohammad | |
| dc.contributor.author | Abdolvahabi, Zohreh | |
| dc.contributor.author | Bolandghamtpour, Zahra | |
| dc.contributor.author | Hesari, Zahra | |
| dc.contributor.author | Yousefi, Zeynab | |
| dc.contributor.author | Panahi, Ghodratollah | |
| dc.contributor.author | Meshkani, Reza | |
| dc.date.accessioned | 2020-03-06T14:12:42Z | |
| dc.date.available | 2020-03-06T14:12:42Z | |
| dc.date.issued | 2019-09-12 | |
| dc.description.abstract | Breast cancer (BC) is the most preval ent cause of cancer-related death in women worldwide. BC is frequently associated with elevated levels of nicotinamide phosphoribos yltransferase (NAMPT) in blood and tumor tissue. MicroRNA-494 (miR-494) has been described to play key anti-tumor roles in human cancers. The aim of the present study was to investigate the inhibitory effect of miR-494 on NAMPT-mediated viability of BC cells. In this experimental study, MCF-7 and MDA-MB-231 cells were cultured and then transfec ted with miR-494 mimic, miR-494 inhibitor and their negative controls. The mRNA and protein expression of NAMPT were assessed using real-time PCR and Western blotting, respectively. Subseq uently, intracellular NAD levels were determined by a colorimetric method. Finally, cell apoptosis was examined by fl ow cytometry. Bioinformatics evaluations pre- dicted NAMPT as a miR-494 target gene which was confirmed by luciferase reporter assay. Our results showed an inverse relationship between the expression of miR-494 and NAMPT in both MCF-7 and MDA-MB-231 cell lines. miR-494 significantly down-regulated NAMPT mRNA and protein expression and was also able to reduce the cellular NAD content. Cell viability was decreased fo llowing miR-494 up-regulation. In addition, apoptosis was induced in MCF-7 and MDA-MB-231 cells by miR-494 mimic. Our findings indicate that miR-494 acts as a tumor suppressor and has an important effect in suppressing the growth of BC cells through NAMPT. Therefore, miR-494 might be considered as a novel therapeutic target for the management of human breast cancer. | en |
| dc.identifier.issn | 1611-2156 | |
| dc.identifier.uri | http://hdl.handle.net/2003/39052 | |
| dc.identifier.uri | http://dx.doi.org/10.17877/DE290R-20971 | |
| dc.language.iso | en | |
| dc.relation.ispartofseries | EXCLI Journal;Vol. 18 2019 | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Breast cancer | en |
| dc.subject | NAMPT | en |
| dc.subject | Apoptosis | en |
| dc.subject | miR-494 | en |
| dc.subject | microRNA | en |
| dc.subject.ddc | 610 | |
| dc.title | MicroRNA-494 induces breast cancer cell apoptosis and reduces cell viability by inhibition of nicotinamide phosphoribosyltransferase expression and activity | en |
| dc.type | Text | |
| dc.type.publicationtype | article | |
| dcterms.accessRights | open access | |
| eldorado.dnb.zdberstkatid | 2132560-1 | |
| eldorado.secondarypublication | true |