Roselle attenuates cardiac hypertrophy after myocardial infarction in vivo and in vitro
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Date
2019-09-26
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Abstract
Roselle (Hibiscus sabdariffa Linn) has been traditionally used as folk medicine for hypertension
and maintaining cardiovascular health, with therapeutic potential in protecting against numerous
cardiovascular diseases. However, it remains unclear whether roselle can be used for management of
cardiac hypertrophy seen after myocardial in- farction (MI). This study therefore investigated the
effects of aqueous roselle extract on cardiac hypertrophy aris- ing from myocardial infarction both
in vivo and in vitro. For in vivo study, male Sprague-Dawley rats were divided into control or MI
groups (receiving 85 mg/kg isoproterenol s.c. for 2 days) and were given roselle extract (100
mg/kg, p.o daily) for 28 days. Cardiac structure and functional changes were evaluated at study
end-point using histology, Langendorff analysis and gene expression analysis. In vitro effects of
roselle were also assessed on ANG II-induced cardiomyocytes hypertrophy using H9c2 cells,
simulating cardiac hypertrophy evident after MI. Roselle significantly ameliorated MI-induced
cardiac systolic and diastolic dysfunction, as seen across improve- ment in left ventricular
developed pressure (LVDP) and its derivative (LVdP/dtmax) and isovolumic relaxation (Tau).
Oxidative stress evident across elevated pro-oxidant markers (NOX2 subunit of NADPH oxidase and 8-
isoprostane) as well as reduced antioxidant markers (superoxide dismutase and glutathione) were
also significantly attenuated by roselle. Furthermore, roselle treatment markedly reduced markers
of cardiac remodeling (cardiac hypertrophy and fibrosis) compared to the untreated MI rats. On in
vitro analysis, roselle significantly attenuated ANG II-induced cardiomyoycte hypertrophy in
dose-dependent manner. This study demonstrated that roselle at- nd dysfunction seen after MI both
in vivo and in vitro, and these effects are likely
mediated by phenolic compounds found in roselle extract.
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Keywords
Cardiac dysfunction, Cardiomyocyte hypertrophy, Fibrosis, Myocardial infarction, Oxidative stress, Roselle