Roselle attenuates cardiac hypertrophy after myocardial infarction in vivo and in vitro

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dc.contributor.authorSi, Lislivia Yiang-Nee
dc.contributor.authorRamalingam, Anand
dc.contributor.authorAli, Shafreena Shaukat
dc.contributor.authorAminuddin, Amnani
dc.contributor.authorNg, Pei-Yuen
dc.contributor.authorLatip, Jalifah
dc.contributor.authorKamisah, Yusof
dc.contributor.authorBudin, Siti Balkis
dc.contributor.authorZainalabidin, Satirah
dc.date.accessioned2020-03-06T14:21:58Z
dc.date.available2020-03-06T14:21:58Z
dc.date.issued2019-09-26
dc.description.abstractRoselle (Hibiscus sabdariffa Linn) has been traditionally used as folk medicine for hypertension and maintaining cardiovascular health, with therapeutic potential in protecting against numerous cardiovascular diseases. However, it remains unclear whether roselle can be used for management of cardiac hypertrophy seen after myocardial in- farction (MI). This study therefore investigated the effects of aqueous roselle extract on cardiac hypertrophy aris- ing from myocardial infarction both in vivo and in vitro. For in vivo study, male Sprague-Dawley rats were divided into control or MI groups (receiving 85 mg/kg isoproterenol s.c. for 2 days) and were given roselle extract (100 mg/kg, p.o daily) for 28 days. Cardiac structure and functional changes were evaluated at study end-point using histology, Langendorff analysis and gene expression analysis. In vitro effects of roselle were also assessed on ANG II-induced cardiomyocytes hypertrophy using H9c2 cells, simulating cardiac hypertrophy evident after MI. Roselle significantly ameliorated MI-induced cardiac systolic and diastolic dysfunction, as seen across improve- ment in left ventricular developed pressure (LVDP) and its derivative (LVdP/dtmax) and isovolumic relaxation (Tau). Oxidative stress evident across elevated pro-oxidant markers (NOX2 subunit of NADPH oxidase and 8- isoprostane) as well as reduced antioxidant markers (superoxide dismutase and glutathione) were also significantly attenuated by roselle. Furthermore, roselle treatment markedly reduced markers of cardiac remodeling (cardiac hypertrophy and fibrosis) compared to the untreated MI rats. On in vitro analysis, roselle significantly attenuated ANG II-induced cardiomyoycte hypertrophy in dose-dependent manner. This study demonstrated that roselle at- nd dysfunction seen after MI both in vivo and in vitro, and these effects are likely mediated by phenolic compounds found in roselle extract.en
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/2003/39055
dc.identifier.urihttp://dx.doi.org/10.17877/DE290R-20974
dc.language.isoen
dc.relation.ispartofseriesEXCLI Journal;Vol. 18 2019
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCardiac dysfunctionen
dc.subjectCardiomyocyte hypertrophyen
dc.subjectFibrosisen
dc.subjectMyocardial infarctionen
dc.subjectOxidative stressen
dc.subjectRoselleen
dc.subject.ddc610
dc.titleRoselle attenuates cardiac hypertrophy after myocardial infarction in vivo and in vitroen
dc.typeText
dc.type.publicationtypearticle
dcterms.accessRightsopen access
eldorado.dnb.zdberstkatid2132560-1
eldorado.secondarypublicationtrue

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