Genome research is emerging as a new and important tool in biology used to obtain information on gene sequences, genomic interaction, and how genes work in concert to produce the final syndrome or phenotype. Defect in phenylalanine hydroxylase (PAH) gene result in Phenylketonuria (PKU). Molecular studies using the brain of the mouse model for PKU (PAHenu2) showed altered expression of several genes including upregulation of orexin A and a low activity of branched chain aminotransferase. These...
Emission of volatile organic substances (VOC) from articles inside a car may lead to adverse health effects in exposed drivers. Presently, no general concept to derive maximum exposure levels inside cars has been published. Therefore, we recommend techniques for three types of maximum exposure levels inside cars, namely for (i) chronic exposure to non-genotoxic substances (ELIA, chronic), (ii) short term exposure inside automotive vehicles (STELIA) and (iii) genotoxic substances acting by thr...
The expression of extracellular matrix (ECM) specific receptors, integrins, was investigated in two closely related strains of oncogenically transformed fibroblasts drastically differing in spontaneous metastasizing. The highly metastatic cells (HM) were shown to express in their surface membranes much higher levels of collagen/vitronectin-specific integrin avß3, as compared to their lowly metastatic counterparts (LM). Inhibition of the avß3-mediated signal transduction strongly reduced the i...
The Severe and Acute Respiratory Syndrome coronavirus (SARS CoV) is a newly-emerged virus that caused an outbreak of atypical pneumonia in the winter of 2002-2003. Polyclonal antibodies raised against the nucleocapsid (N) of the SARS CoV showed the localization of N to the cytoplasm and the nucleolus in virus-infected and N-expressing Vero E6 cells. Like other coronavirus N proteins, the SARS N is probably a phosphoprotein. N protein expressed in mammalian cells is apparently able to "spread"...
Cellular prion protein, PrP^C, undergoes pathogenic structural conversion into the proteinase K (PK)-resistant isoform, PrP^Sc, to constitute a nucleic acid-free infectious agent, so called a prion. To determine whether a recently identified PrP-like protein, named PrPLP/Dpl, could also be transformed to a prion-like protein, we intracerebrally inoculated a mouse-adapted Fukuoka-1 prion into Ngsk and Zrch I mice either homozygously (Prnp^0/0) or heterozygously (Prnp^0/+) devoid of PrP^C. Only...
The aim of the study was to investigate the role of integrins in anchorage dependent apoptosis (anoikis) and in vitro invasion of human breast cancer cell line MCF-7 and its multidrug resistant subline MCF-7Dox. Acquisition of MDR was associated with markedly decreased expression of collagen specific a2ß1 and avß3 integrins, laminin specific a3ß1 and a6ß1 receptors and dramatic up-regulation of fibronectin specific a5ß1 integrin. The MDR subline was substantially more resistant to anoikis tha...
A recent clinical study has focused on: 1- the interaction between genetic variants of glutathione S-transferases M1 and T1 (GSTM1 and GSTT1) and smoking on the risk of cardiovascular diseases, 2- the potential capacity of GSTM1 and T1 genotypes in modifying the effect of smoking on inflammation and endothelial function. In this study, we investigated whether carriage of these 2 polymorphisms altered the smoking impact on biological indices of inflammation and cellular adhesion. White blood c...
Even though many scales for the assessment of sexual dysfunction have been recently developed, most of them are suitable rather for the research purpose in clinical trials than to routine interviews in a daily, private practice. We report here the first experience with a simple, semi-quantitative scale for parallel assessment of sexual dysfunction and life-satisfaction (considered to globally reflect the quality of life), which was tested in depressed patients treated in the psychiatric, priv...
In a previous study we presented a technique to differentiate between randomly predictable and randomly unpredictable amino acid pairs. We have shown that all of 95 variants in human collagen alpha1(I) chain precursor (CA11) occurred at randomly unpredictable amino acid pairs. To study whether this is unique for the human collagen alpha1(I) chain precursor (CA11) or whether this is a more general principle we examined another human collagen precursor, the human collagen alpha1(III) chain prec...
In this data-based theoretical analysis, we use our random approach to analyse amino acid pairs in human collagen a 1(I) chain precursor (CA11) in order to determine which amino acid pairs are more sensitive to 95 variants with missense mutant in human CA11 protein. The rationale of this study is based on our hypothesis and previous findings that harmful variance is more likely to occur at randomly unpredictable amino acid pair position rather than at randomly predictable positions. This is r...
Overt hypothyroidism frequently leads to liver dysfunction. Subclinical hypothyroidism (SCH) is linked to abnormalities of lipoprotein metabolism, however, data on liver function are lacking. We analyzed the effects of L-thyroxine (L-T4) therapy on liver function and their association with changes of serum lipoproteins in SCH as part of a prospective, double-blind study. 66 women with SCH were randomly assigned to receive either L-thyroxine or placebo for 48 weeks. Circulating liver and bilia...
- M.-A. von Mach
- J. G. Hengstler
Leibniz Research Centre for Working Environment and Human Factors
- L. S. Weilemann, Mainz, Germany
- I. Ishibashi, Tokyo, Japan
- P. Micke, Uppsala, Sweden
- J. Timmer, Freiburg, Germany
- A. Winterpacht, Erlangen, Germany
- B. C. Behera, Pune, India
Address & Contact:EXCLI
Mrs. S. Lindemann
Institute for Occupational Physiology
at the University of Dortmund
Leibniz Research Centre
for Working Environment and Human Factors